摘要
以陇东地区野生紫花苜蓿无菌苗的下胚轴、子叶、叶片和叶柄为外植体,MS为基本培养基,研究划破种皮以及不同生长调节物质种类与配比对愈伤组织诱导和分化影响的结果表明:划破种皮可提高种子发芽率;在外植体中下胚轴的愈伤组织诱导率最高,达92.2%;最佳愈伤组织诱导培养基为MS+2,4-D2.0mg·L-1(单位下同)+NAA1.0+2.5%蔗糖+0.6%琼脂,最佳分化诱导培养基为MS+KT0.5+NAA0.3+2.5%蔗糖+0.6%琼脂,成苗培养基为1/2MS+NAA0.1+1%蔗糖+0.6%琼脂。
Hypocotyls, cotyledons, leaves and petioles from aseptic seedlings of Medicago sativa L. in Longdong region were used as explants in this study. The basic medium was MS solid medium. The effects of seed coat scratching as well as different growth regulators and their combinations on induction and differentiation of embryogenic callus were observed. The result indicated that seed coat scratching had remarkable influence on seeds germination ratio. The downside of hypocotyl possessed the highest callus induction ratio of 92.2% among all materials. The optimal callus induction medium was MS＋2,4-D 2.0 mg·L^-1 （same hereinbelow）＋NAA 1.0＋2.5% sucrose＋0.6% agar, the optimal differentiation induction medium was MS＋KT 0.5＋NAA 0.3＋2.5% sucrose＋0.6% agar, and the optimal seedling formation medium was l/2MS＋NAA 0.1＋1% sucrose ＋0.6% agar.
出处
《植物生理学通讯》
CSCD
北大核心
2009年第12期1177-1180,共4页
Plant Physiology Communications
基金
国家“十一五”科技支撑计划(2007BAD52B06
2006BAD01A19-6)
国家牧草产业技术体系项目(2008)
关键词
陇东地区野生紫花苜蓿
胚性愈伤组织
诱导
再生
Medicago sativa L. in Longdong region
embryogenic callus
induction
regeneration
