摘要
目的:通过建立实验性自身免疫性前列腺炎(EAP)大鼠模型,探讨IL-10对EAP的治疗作用及对肿瘤坏死因子-α(TNF-α)和转化生长因子-β1(TGF-β1)表达的影响。方法:30只Wistar大鼠随机分为正常对照组(C组,10只)、EAP模型阳性对照组(P组,10只)和IL-10干预组(I组,10只)。C组给予生理盐水,P组以Wistar大鼠前列腺蛋白提纯液辅以双重免疫佐剂制作EAP模型,I组在诱导EAP模型后应用IL-10干预。HE染色观察各组前列腺组织炎性细胞浸润情况,电镜观察前列腺细胞及细胞周围超微结构的变化,半定量RT-PCR法检测前列腺组织TNF-α和TGF-β1的含量。结果:P组前列腺组织的炎症程度、TNF-α和TGF-β1水平明显高于C组(P<0.05),I组大鼠经IL-10治疗后前列腺组织炎性细胞浸润减轻,TNF-α和TGF-β1水平较P组下降,差异均有显著性(P<0.05)。结论:IL-10可减轻EAP大鼠前列腺组织炎症浸润,降低TNF-α和TGF-β1的表达,对EAP有一定的治疗作用。
Objective： To study the efficacy of interleukin-10 （IL-10） in the treatment of experimental autoimmune prostatitis （EAP） and its effect on the expressions of tumor necrosis factor-α （TNF-α） and transforming growth factor-β1 （TGF-β1） in EAP rat models. Methods： Thirty Wistar rats were equally and randomly divided into a control, an EAP and an IL-10 group. The controls were treated with normal saline, the EAP models were made by injection of purified prostate protein twice with immune adjuvant, and the IL-10 group included the EAP models subjected to IL-10 intervention. The infiltration of the inflammatory cells of the prostate tissue was detected by HE staining, the ultrastructure of the prostate cells and their surrounding cells observed by electron microscopy, and the levels of TNF-α and TGF-β1 in the three groups determined by semi-quantitative RT-PCR assay. Results： The EAP group showed sig- nificantly severer inflammation and higher levels of TNF-α and TGF-β1 in the prostate tissue than the controls （P 〈 0.05）. The IL-10 group exhibited significantly lessened inflammatory infiltration of the prostate tissue and decreased levels of TNF-α and TGF-β as compared with the EAP group （P 〈 0.05）. Conclusion： IL-10 could relieve inflammatory infiltration of the prostate tissue and inhibit the expressions of TNF-α-and TGF-β1 in EAP rats, which is suggestive of its therapeutic efficacy for autoimmune prostatitis.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2009年第12期1085-1089,共5页
National Journal of Andrology
基金
浙江省卫生厅基金项目(2008A122)
浙江省医学会基金项目(2008ZYC23)
浙江省温州市龙湾区科技局基金项目(2008S3)~~
