摘要
目的:筛选并优化嗜热脂肪芽孢杆菌CHB1液体发酵培养基。方法:通过Plackett-Burman试验和响应面分析方法,确定培养基的主要影响因素和最佳浓度。结果:利用SAS软件进行分析,确定对响应值影响最大的3个因素为豆粕、酵母粉、K2HPO4。最佳培养基组成为0.51%、豆粕浓度为0.425%、K2HPO4浓度为0.994%。根据模型预测得到的理论最大菌数为2.94×108cfu。在初始条件下实验,菌数为2.40×108cfu,在优化的最佳培养基条件下,实际的菌数为3.06×108cfu。菌数比优化前提高了27.3%。试验值与预测值的误差为4.08%。结论:实验值与模型预测值拟合良好。
Objective： Screened and optimized the liquid fermentation medium of Geobacillus stearothermophilus.Method： Plackett-Burman experiment design and response surface method were used to study the fermentation medium.Result： SAS software was used to design and analyze the three factors and three levels of response surface analysis experimentation.The results showed that the best medium is yeast extract 0.51%,soybean meal 0.425%,K2HPO4 0.994%.The largest number of bacteria predicted by the theoretical model is 2.40×10^8 cfu.Bacterial counts for the 2.40×10^8 cfu under the conditions of initial fermentation medium.After fermentation medium optimization,the actual number of bacteria increased 27.3 percent reached 3.06×10^8 cfu.The error of test value and predictive value is 4.08%.Conclusion：The experimental data and the predicted value of model fit well.
出处
《生物技术》
CAS
CSCD
北大核心
2009年第6期66-69,共4页
Biotechnology
基金
福建省科技重点项目(2006I0009)
福建省农业科技创新团队建设基金(STIF-Y01)资助
