摘要
目的：借助体外模型验证IRF-1、CD74、CD36、GAP43、CYP2E1和TCA4基因表达变化是否与糖尿病大血管病变相关。方法：取自发性高血压大鼠胸主动脉平滑肌细胞，并将其分别培养于含不同浓度葡萄糖（5．5、15、25、30mmol／L和35mmol／L）、波动糖中（25mmol／L和5．5mmol／L葡萄糖交替，每一浓度持续12h）以及持续高糖（25mmol／L）中，48h后运用半定量RT—PCR检测IRF-1、CD74、CD36、CYP2E1和TCA4的表达变化，SPSS分析基因变化与平滑肌增殖的相关性。结果：葡萄糖浓度低于30mmol／L时，平滑肌细胞的增殖随糖浓度的增加而加速。持续高糖中培养平滑肌细胞自第6h起增殖明显。统计分析显示，CD74、IRF-1和GAP43的表达变化与平滑肌细胞的增殖存在相关性。结论：IRF-1和CD74参与调节糖尿病大血管病变中平滑肌细胞的增殖。
Objective： Our previous study exhibited that IRF-1, CD74, CD36, GAP43, CYP2E1 and TCA4 upregulated their expression to respond for diabetic macroangiopathy. This study was performed to identify whether these genes correlate to diabetic macroangiopathy based on in vitro model. Methods： Macrovascular smooth muscle cells isolated from spontaneously hypertensive rats were cultured in different dose glucose （5.5 mmol/L, 15 mmol/L, 25 mmol/L, 30 mmol/L, 35 mmol/L, a cyclically variated dose of 25 mmol/L and 5.5 mmol/L at 12-h intervals） or unchanged 25 mmol/L glucose media for 48 h. Semi quantitative RT-PCR was applied to detect the expression of genes abovementioned. Correlation of alteration of genes expression to VSMCs survival was analyzed statistically. Results： Proliferative viability of macrovascular smooth muscle cells was enhanced following the glucose increase below 30 mmol/L, and declined at 35 mmol/L. VSMCs presented obvious proliferative phenotype after 6 h culture in unchanged 25 mmol/L glucose and accelerated thereafter. CD74, IRF-1 and GAP43 altered their expression statistically responding for the proliferation of VSMCs. Conclusion： IRF1 and CD74 may participate in regulating the prolifera tion of VMSCs in diabetic macroangiopathy.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2009年第6期731-735,共5页
Chinese Journal of Anatomy
基金
Natural Science Foundation of Shanshai Municipal Goverment （032R14101） and National Natural Science Foundation of China （30570760）
关键词
干扰素调节因子-1
CD74
增殖
大血管平滑肌细胞
高糖
基因表达
interferon regulatory factor-1
CD74
proliferation
macrovascular smooth muscle cells
high glucose
gene expression
