摘要
目的探讨葡萄籽原花青素(Grape seed proanthocyanidin extract,GSPE)对大鼠急性肾缺血再灌注损伤的保护作用及其机制。方法32只SD大鼠随机分为4组,每组8只,分别为假实验组(SG),缺血再灌注组(I/RG),急性GSPE(450mg/kg灌胃1次)治疗+I/R组(ATG),慢性GSPE(150mg/kg灌胃喂养1周)治疗+I/R组(CTG)。采用一侧肾蒂结扎、另一侧肾动脉夹闭的肾缺血再灌注损伤模型,测定肾组织丙二醛(MDA)水平和超氧化物歧化酶(SOD)的活性,同时测定血清尿素氮(BUN)和肌酐(Cr)水平。结果I/RG与SG相比,MDA、BUN和Cr含量明显升高(P<0.05),SOD活性下降(P<0.05)。与I/RG相比,ATG的MDA和Cr含量下降(P<0.01,P<0.05),CTG的MDA、BUN和Cr含量明显下降(P<0.05,P<0.01),SOD活力显著升高(P<0.05)。结论葡萄籽原花青素对肾缺血再灌注损伤具有保护作用,其机制可能与抗自由基损伤有关。
Objective To investigate the protective effects and mechanism of grape seed proanthocyanidin extract （GSPE） on renal ischemic reperfusion injury in rats.Methods 32 rats were randomly divided into 4 groups.8 rats were used in each group,including sham operation group,ischemic reperfusion group,acute GSPE treatment＋I/R group （GSPE 450 mg/kg once by gavage）and chronic GSPE treatment ＋I/R group（GSPE 150mg/kg by gavage for a week）.Using the model with one side of renal ligation,the other side of the renal artery occlusion renal ischemia-reperfusion injury,Malondialdehyde （MDA） levels and superoxide dismutase （SOD） activities were determined to assess lipid peroxidation.BUN and Cr were also determined to assess the function of the kidney.Results Compared with SG group,the levels of MDA,BUN and Cr were significantly higher （P〈0.05）,SOD activity decreased （P〈0.05） in I/RG.Compared with I/RG,MDA and Cr content of ATG decreased （P〈0.01,P〈0.05）,MDA,BUN and Cr of CTG decreased significantly（P〈0.05,P〈0.01）,SOD activity significantly higher （P〈0.05）.Conclusion The grape seed proanthocyanidins has a protective effect on renal ischemia-reperfusion injury,which may be related to anti-free radical damage.
出处
《实用药物与临床》
CAS
2009年第6期387-389,共3页
Practical Pharmacy and Clinical Remedies
关键词
葡萄籽原花青素
肾脏
缺血再灌注损伤
Grape seed proanthocyanidin extract
Kidney
Ischemia reperfusion injury
