摘要
目的探讨双歧杆菌脂磷壁酸(LTA)对小鼠腹腔巨噬细胞体外吞噬功能和NO释放的影响。方法将无菌收集培养的小鼠腹腔巨噬细胞分为RPMI 1640完全培养液组和地塞米松处理组(终浓度为100μg/ml),分别向每组培养孔内加入不同终质量浓度的LTA,于培养4 h和24 h后,分别用中性红法和Griess Reagent试剂盒检测并分析LTA对巨噬细胞体外吞噬和NO释放的影响。结果终浓度为5、10、20、40μg/ml的LTA对小鼠腹腔巨噬细胞体外吞噬功能和NO的释放均有明显的促进作用(P<0.05),并且可以有效拮抗地塞米松的抑制效应(P<0.05)。LTA的上述作用与其浓度呈一定的梯度关系。结论LTA不但可以显著激活小鼠腹腔巨噬细胞的体外吞噬功能和促进NO的释放,还能有效拮抗地塞米松对巨噬细胞的体外抑制作用。
Objective To explore the effects of lipoteichoic acid（LTA）on phagocytosis of peritoneal macrophages and NO production in vitro.Methods The peritoneal macrophages that were isolated sterilely from Kunming mice were divided into two groups：co-culture with RPMI 1640,and pretreatment with dexamethasone（DEX）.Then,the LTA isolated from BFA with different final concentrations（5,10,20 and 40 μg/ml）was added.After the peritoneal macrophages were cultured for 4 and 24 h,the effects of LTA on cytophagocytesis and NO production in vitro were determined respectively by using neutral red and NO assay kit.Results LTA with final concentrations of 5,10,20 and 40 μg/mL significantly promoted the phagocytosis of peritoneal macrophages and production of NO（P〈0.05）.It also effectively antagonized the inhibitory response of DEX against peritoneal macrophages（P〈0.05）.The higher concentration of LTA,the stronger effect.Conclusion Not only can LTA stimulate and activate the peritoneal macrophages significantly,but also antagonize the inhibition from DEX in vitro.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2009年第6期812-814,共3页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词
脂磷壁酸
青春双歧杆菌
地塞米松
巨噬细胞
吞噬作用
NO释放
lipoteichoic acid
adolescentis
dexamethasone
peritoneal macrophages
phagocytosis
NO production
