摘要
提出了一条活性干酵母中提取海藻糖的工艺路线。通过控制抽提温度、乙醇浓度以及抽提时间,得出较佳的提取工艺条件。用阴阳离子交换柱去除杂质,同时起到一定的脱色作用,进而运用超滤进行澄清。最后经浓缩、结晶以及干燥,制成海藻糖产品。其得率可达:每100g活性干酵母产14.5g海藻糖。
In this paper a method of producing trehalose from yeasts was proposed. The optimum extracting conditions were obtained, which include temperature,alcohol concentrtion and extracting time. The extract was decolored and desalted by using anion and cation exchange resin.The desalted liquid was filtered with an ultrafilter membrane. At last,the permeate was concentrated and crystalized with alcohol and then separated by centrifugation of Bushfilter. The crystals were obtained with a yield of 14.5 g trehalose(including two H 2O)/100 g dried active yeasts.
关键词
酵母
海藻糖
提取
超滤澄清
制糖
baker's yeast
trehalose
extraction
ultrafiltration