摘要
通过Cu2+与谷胱甘肽(GSH)的配位作用,在过量Cu2+溶液中,利用循环伏安法测定剩余铜的阳极溶出峰电流的方法,间接测定了GSH的含量.实验表明,铜的氧化峰电流随溶液中GSH浓度的增加呈线性降低.用该方法测定7次的相对标准偏差(RSD)为0.513 2%,检测限达5.6×10-4mmol/L.利用该方法测定针剂中的GSH含量,并与光度法的测定结果相比较,两者结果基本吻合.本研究提供了一种简便、可靠、快速地测定基体成分不复杂样品中GSH含量的分析方法.
We discribe a indirect determination method of glutathione（GSH） using cyclic voltammetric determi nation according to the eomplexation interaction between copper ion and GSH. In the extra copper ion solutions, anodic stripping currents of copper is linearly decreased as the GSH concentration increased. The method is reproducible and sensitive with RSD 0. 513 2% （7 paralel determination） and detection limit 5. 6 × 10 ^-4 mmol/L. Satisfactory results were obtained by the determination of GSH in the injection sample and the results is correspondant with the result determined by UV-Vis spectrometric methods.
出处
《延边大学学报（自然科学版）》
CAS
2009年第4期345-349,共5页
Journal of Yanbian University（Natural Science Edition）
关键词
谷胱甘肽
循环伏安法
铜
电化学分析
glutathione
cyclic voltammetry
copper
electroanalysis
