摘要
目的建立非体外循环下犬白细胞滤除法。方法健康成年杂种犬21只，雌雄不拘，体重10～12kg，随机分为2组，对照组（C组，n=9）和白细胞滤除组（LD组，n=12）。行双侧颈外静脉穿刺置管，建立白细胞过滤端口。LD组将右侧颈外静脉导管口作为动脉端，通过MP-300输血导管与SQ40S白细胞过滤器回流至左侧颈外静脉导管口，形成滤除白细胞的闭合回路。肝素抗凝后，采用MP-300输血导管滚动泵作为血液驱动的动力，以75ml／min的速率滤除白细胞，持续60min，随后关闭右侧颈外静脉导管端口，向滤除白细胞的闭合回路中注入生理盐水，通过滚动泵将残余血液泵入体内，关闭左侧颈外静脉端口。C组仅行双侧颈外静脉穿刺置管。细于白胞滤除前即刻（基础状态，To）、滤除10、20、30、40、50、60min（T1-6，滤除期）、滤除结束后10、20、30、40、50、60、120、180和270min（T72-15恢复期）时抽取动脉血样0．5ml，测定血常规，于T0、T6、T11、T15时记录平均动脉压（MAP）、心率（HR）、红细胞计数和血小板计数和体温。结果与C组比较，LD组MAP、红细胞计数差异无统计学意义（P〉0．05），T6时HR、T6，11时体温、R6,11，15时血小板计数降低、滤除期白细胞浓度降低（P〈0．05），恢复期两组白细胞浓度差异无统计学意义（P〉0．05）。结论本实验成功建立了非体外循环下犬白细胞滤除法，其滤除白细胞的效果确切，不良反应少。
Objective To establish a off-pump leukocyte depletion method in canines. Methods Twenty- one healthy adult mongrel dogs of both sexes, weighing 10-12 kg, were randomized into 2 groups： control group （group C, n = 9） and leukocyte depletion group （group LD, n = 12）. An extracolporeal leukocyte filtration end was constructed by aseptic puncture of the bilateral external jugular veins via a blood transfusion line and the leukocyte filter for extracorporeal leukocyte depletion. In group LD, blood was filtered with a MP-300 blood line and a SQ40S leucocyte depletion filter one end placed in the right external jugular vein （ artery end） and the other end placed in the left external jugular vein （venous end ）. After heparin antieoagulation, a MP-300 blood line pump was used as the power. Blood was filtered at a rate of 75 ml/min and it was maintained for 60 rain. The artery end was then closed, normal saline injected into the closed circuit, the remaining blood pumped into the body, and then the venous end closed. In group C, aseptic puncture of the bilateral external jugular veins was performed. Arterial blood samples were taken immediately before leukodepletion （baseline, T0）, at 10, 20, 30, 40, 50 and 60 rain of leukodepletion （T1-6 , depletion period）, and at 10, 20, 30, 40, 50, 60, 120, 180 and 270 min after leukodepletion （T715 , recovery period） for determination of blood routine. MAP, HR, RBC, Pit and body temperature were recorded at T0 , T6 , T12 and T15 . Results There were no significant difference in MAP and RBC between the two groups （ P 〉 0.05 ）. HR, body temperature, and Pit were significantly lower in group LD than in group C （P 〈 0.05）. The leukocyte concentration was lower during depletion period in group LD than in group C （P 〈 0.05）, while there was no significant difference in leukocyte concentration during recovery period between the two groups （ P 〉 0.05 ）. Conclusion The off-pump leukocyte depletion method is successfully established and has
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2009年第11期1005-1007,共3页
Chinese Journal of Anesthesiology
