摘要
目的探讨亚低温治疗对重症颅脑损伤患者淋巴细胞中热休克蛋白(HSP)60、HSP70、HSP90表达的影响。方法23例重症颅脑患者随机分为亚低温组(12例)和常温组(11例),两组在常规治疗的基础上,亚低温组给予控温毯降温治疗4～6d,保持体温在32～34℃。治疗后第1d、4d、8d应用WesternBlot方法检测淋巴细胞中HSP60、HSP70、HSP90的表达;治疗7d后观察两组的感染发生率;并观察正常对照者血淋巴细胞在32℃及37℃时HSPs的表达。结果亚低温组各时间点的HSP60表达显著低于常温组(均P<0.05);HSP70、HSP90表达两组间差异无统计学意义;亚低温组感染发生率(66.7%)较常温组(18.2%)显著增加(P<0.01);正常对照组淋巴细胞在32℃条件下HSP60的表达显著低于37℃时(P<0.05),而不同温度条件下HSP70、HSP90的表达差异无统计学意义。结论亚低温治疗显著降低颅脑外伤患者HSP60的表达,抑制机体免疫能力。
Objective To explore the influence of mild hypothermia therapy on expression of heat shock protein （HSP） 60, HSP70, HSP90 in lymphocyte in patients with severe craniacerebral injury. Methods 23 patients with severe craniacerebral injury were randomly divided into the mild hypothermia group （ 12 cases） and the normothermia gToup（ 11 cases）. At the base of routine therapy, the mild hypothermia group received mild hypothermia therapy by the temperature control blanket to keep the body temperature among 32 - 34℃ for 4 - 6 d. The expression of HSP60, HSP70,HSP90 in lymphocyte were measured by Western Blot at the 1st d, 4th d and 8th d after treatment. The infection incidence was observed at 7 d after treatment. And the expression of HSPs in lymphocyte at 32℃ and 37℃ in normal control group were measured respectively. Results Compared with normothermia group, the expression of HSP60 in mild hypothermia group was significantly lower at each time point（ all P 〈0. 05 ）, but the differences of the expression of HSP70 and HSP90 between the two groups were no statistical significance. The infection incidence in mild hypothermia group（66.7% ） was significantly higher than that in normothermia group（ 18.2% ） （P 〈 0.01 ）. In the normal ,Jontrol group, the expression of HSP60 at 32℃ was significantly lower than that at 37℃ （P 〈 0. 05 ） ; while the expression of other HSPs at different temperature had no significant difference. Conclusion Mild hypothermia therapy can obviously decrease the expression of HSP60, thus inhibit the immunity system in patients with severe craniacerebral injury.
出处
《临床神经病学杂志》
CAS
北大核心
2009年第6期404-406,共3页
Journal of Clinical Neurology
基金
吴阶平医学基金(320.2720.0501)
关键词
亚低温
颅脑外伤
热休克蛋白
mild hypothermia
severe craniacerebral injury
heat shock protein
