摘要
目的构建磷灰石晶体矿化的体外模型,研究牛血清白蛋白(BSA)和不同浓度的氟离子对磷灰石晶体体外矿化的影响。方法利用阳离子膜和透析膜建立磷灰石晶体矿化的体外模型。在模型中分别加入去离子水、BSA和不同浓度的氟离子(5、20、100mg·L-1),反应3d后,扫描电镜(SEM)、X线衍射法检测矿化产物。结果成功构建了磷灰石晶体体外矿化模型,加入去离子水和BSA后,晶体成分主要为磷酸八钙(OCP),但晶体形态差异显著。加入氟离子后,形成了柱状、杆状的氟磷灰石(FAP)晶体,且随着氟离子浓度的增加,晶体的尺寸和矿化程度均增加。结论该体外模型是检测磷灰石晶体矿化调控因素的有效方法。
Objective To establish an in vitro model for the apatite crystal mineralization. To evaluate the influences of bovine serum albumin （BSA） and fluoride to the mineralization of apatite crystal. Methods The model was constructed using cation selective membrane （CMV） and dialysis membrane. Double distilled water（DDW）, BSA, 5, 20, 100 mg·L^-1 fluoride were added into the reaction space of the model. Reaction was carried out at 37 ℃ for 3 days under gentle stirring. The crystals were identified by scanning electron microscope （SEM） and X-ray diffraction （XRD）. Results The model was established successfully. When DDW and BSA were added respectively, the main component of the deposit was octacalcium phosphate （OCP）, but the shape and size of the crystals differs from each other. When fluoride with different concentration were added, the main component of the crystal turned to rod-like and prism-like fluoroapatite （FAP） crystal. The size and crystallinity of the FAP increased with the increase of the fluoride concentration. Conclusion It is an effective way to evaluate the influence factors of the apatite crystal mineralization by using the in vitro model.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2009年第6期588-591,共4页
West China Journal of Stomatology
关键词
磷灰石
矿化
模型
apatite
mineralization
model
