摘要
利用高效液相色谱-电感耦合等离子体质谱联用技术研究了海带、羊栖菜、紫菜及浒苔等干海产品中的砷含量及其化学形态。实验表明,干海产品中主要砷形态为3种未知砷化物,对3种未知砷化物进行了表征。采用高效液相色谱与飞行时间质谱联用技术对羊栖菜和海带中的3种未知砷化物的分子量,分别为329.0599,483.0738和409.0162,通过对比,确定这3个未知峰分别为3种砷糖类物质:DMA-glycerol ribose,DMA-phosphate ribose和DMA-sulfate ribose。对样品中砷的定量分析结果显示,干海产品中砷总量虽然超出我国国标中关于海产品中砷限量标准规定的20倍以上,但大多数植物性干海产品中主要的砷形态为毒性较低的砷糖类物质DMA-sulfate ribose(U4),其含量约占可提取砷量的51.1%～80.3%,海带中的砷主要以DMA-phosphate ribose(U3)的形式存在,占总可提取砷的48.9%。
Arsenic and its species in dry seafood products（DSPs） Kelp, Sargassum fusiform, Laver, Entero- morpha prolifera, were systematically determined by high performance liquid chromatography-inductively coupled plasma mass spectrometry（HPLC-ICP-MS）. It was found that the of arsenic species in botanic DSPs are＇three unknown arsenicals（ U2, U3 and U4 ） , which are speculated as arsenosugars and take approximate 63.5% 92. 7% of all arsenic species detected. These arsenosugars were indentified as dimethylarsinic （DMA）-glycerol ribose, DMA-phosphate ribose and DMA-sulfate ribose by their accuracy molecular weight 329.0599, 483.0738 and 409.0162, using direct confirmatory of HPLC-TOF-MS. The quantitative analysis of these arsenic species shows that although the total arsenic exceeds the National Standard value, most of the marine plants DSPs are safe to eat. The main component in most marine plants is DMA-sulfate ribose （51.1%-80.3% ）, while the main component of Kelp is DMA-phosphate ribose（48.9%）. The sum of those three arsenosugars was 63.5%-92.7% of peak areas in all arsenic species detected.
出处
《分析化学》
SCIE
CAS
CSCD
北大核心
2009年第12期1738-1742,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金(No.20675021)资助项目
关键词
高效液相色谱
电感耦合等离子体质谱
飞行时间质谱
砷形态
干海产品
砷糖
High performance liquid chromatography, inductively coupled plasma mass spectrometry, time of flight-mass spectrometry, arsenic, speciation, dry seafood products, arsenosugars
