摘要
考察了不同有机溶剂对光果甘草中光甘草定的提取效果,确定了最佳提取溶剂。在单因素实验的基础上,选择超声提取液料比、提取时间和超声功率3因素为自变量,光甘草定提取率为响应值,采用3因素3水平的响应面实验方法,对光果甘草中光甘草定的超声波提取工艺条件进行了优化。结果表明,光甘草定提取的最佳工艺条件为:以乙酸乙酯为溶剂超声提取,液料比23∶1(mL/g)、提取2次、提取温度44℃、提取时间30min、超声功率500W,该条件提取光甘草定的提取率为0.269%。验证实验表明,所得模型方程能较好地预测实验结果。
Effect of different organic solvents for isolation of glabridin from Glycyrrhiza glabra L. has been evaluated and the optimum solvent was obtained. On the basis of single-factor test,the response surface methodology with 3 factors and 3 levels was adopted. The ultrasound-assisted extraction process parameters of glabridin from Glycyrrhiza glabra L. were optimized by the quadratic general spinning design on the ratio of solvent to solid,extraction time and ultrasonic power. The yield of glabridin was used as the responsive values. The results indicate that the optimum conditions for glabridin are as follows： solvent is ethyl acetate, extraction 2 times, ultrasonic power is 500 W, extracting time is 30 min, the ratio of solvent to solid is 23：1 （mL/g）, and extracting temperature is 44 ℃, the maximum yield of glabridin is 0.269%. The validation test shows that the prediction values of the model are well agreed with the experimental values.
出处
《食品科技》
CAS
北大核心
2009年第12期235-239,共5页
Food Science and Technology
关键词
光甘草定
光果甘草
响应面
超声波
glabridin
Glycyrrhiza glabra L
response surface analysis
ultrasound technology
