摘要
建立用毛细管柱气相色谱-质谱法(GC-MS)分析螺旋藻中脂肪酸组成及亚麻酸含量的方法。螺旋藻中的脂肪酸用质谱进行定性确证分析,螺旋藻的脂肪酸组成用总离子流色谱图(TIC)归一化法计算。同时通过TIC使用外标法对螺旋藻中的亚麻酸含量进行定量分析。质谱定性结果表明所检螺旋藻中含有10种不饱和脂肪酸,根据峰面积归一法不饱和脂肪酸占46.774%,而γ-亚麻酸含量高达26.702%。GC-MS定量分析螺旋藻中亚麻酸在50.0～5000.0μg/ml质量浓度范围内有较好的线性关系,线性方程为Y=15843X-533217,R2=0.9986。定量分析结果表明所检螺旋藻中两种亚麻酸总量为396mg/100g,而γ-亚麻酸为393mg/100g。
A gas chromatography-mass spectrometry（GC-MS） method was established to identify compositions of fatty acids and determine the content ofγ-linolenic acid in Spirulina.The compositions of fatty acids in Spirulina were identified through MS and γ-linolenic acid was quantitatively determined by a normalization method of total ion chromatograph.Qualitative analysis results revealed that 10 unsaturated fatty acids were detected and the content of unsaturated fatty acids was 46.774%;meanwhile,the content ofγ-linolenic acid was 26.702%.The determination ofγ-linolenic acid using GC-MS was expressed to be Y = 15843X-533217 with a correlation coefficient of 0.9986.An excellent linear range forγ-linolenic acid was 50.0-5000.0 μg/ml.Total content of linolenic acid in Spirulina was 396 mg/100 g andγ-linolenic acid was 393 mg/100 g.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第22期280-282,共3页
Food Science
