摘要
目的:本研究拟探讨食管癌相关基因2(ECRG2)蛋白产生后在食管癌EC109细胞的分布。方法:利用35S-蛋氨酸标记的Pulse-chase实验示踪ECRG2蛋白产生后在EC109细胞内外的分布情况;利用免疫荧光染色方法观察ECRG2蛋白在EC109细胞内的亚分布。结果:Pulse-chase示踪实验显示ECRG2蛋白产生后,一部分聚集于细胞核内,另一部分被分泌到细胞外;进一步免疫荧光染色分析显示,在间期细胞中ECRG2聚集于中心体处,在有丝分裂期细胞聚集于着丝粒处。结论:ECRG2基因有可能分别在细胞内、细胞外发挥不同的生物学功能,通过细胞外发挥丝氨酸蛋白酶抑制剂功能、细胞内参与中心体复制、纺锤体检测点功能的调控,而在肿瘤细胞侵袭迁移、染色体不稳定性中发挥重要作用。
Objective：To study the distribution of ECRG2 protein in the EC109 cells.Methods：Using pulse-labeled with ／ methionine and Western blotting to examine the distribution of ECRG2 protein in EC109 cells.Using immunofluoscence staining to observe the localization of ECRG2 in EC109 cells.Results：Pulse-chase experiments showed that ECRG2 accumulated in both supernatant and nucleus.Further immunofluoscence staining showed that ECRG2 localized in centrosomes during interphase and kinetochores in mitosis.Conclusion：ECRG2 protein was transferred to both intracellular and extracellular cells,and may involve in regulation of tumor migration/invasion,centrosome duplification and mitotic spindle checkpoint,and play an important role through different mechanisms during migration/invasion and chromosome instability of cancer.
出处
《中国卫生检验杂志》
CAS
2009年第12期2758-2759,共2页
Chinese Journal of Health Laboratory Technology
基金
国家自然科学基金(30500588)
山西省高等学校优秀青年学术带头人项目资助
