摘要
目的:探讨艾灸防治克隆氏病(CD)肠纤维化的作用机制。方法:将60只SD大鼠随机分成正常组、模型组、隔药灸组、温和灸组和西药组,采用三硝基苯磺酸制备大鼠CD模型。隔药灸组与温和灸组选择天枢、气海穴治疗,西药组以柳氮磺胺吡啶溶液灌胃治疗。分别采用ELISA法和HE法观察CD大鼠结肠黏膜TNF-α、TGF-1β含量及组织病理学变化。结果:与正常组比较,模型组TNF-α、TGF-β1含量显著增高,呈现典型的CD肠道炎症和肠纤维化表现;隔药灸、温和灸和西药组则均有明显降低,结肠组织形态结构也有不同程度的改善,且两艾灸组优于西药组。结论:艾灸可能通过降低结肠黏膜TNF-α、TGF-β1的含量,从而减轻CD肠道炎症,防治肠纤维化发生。
Objective：To explore the regulatory mechanisms of moxibustion on the intestinal fibrosis in crohn＇s disease（CD）. Methods： Totally 60 SD rats were randomly divided into model control （MC） group, herbs-partition moxibustion （ HPM ） group, mild-warm moxibustion （ MWM ） group, salicylazosulfapyrldine （SASP） group and normal control （NC） group. The rat models of CD were induced by trinitrobenzene sulfonic acid （TNBs）. The HPM and MWM groups were treated by moxibustion at Tianshu （ST25） and Qihai （RN6）, and the SASP group was fed with SASP solution. The intestinal morphology and TNF-α, TGF-β1 contents were observed by hematoxylin and eosin staining and enzyme linked immunosorbent assay. Results： Compared with the NC group, the contents of TNF-α and TGF-β1 in intestinal mucosa were remarkably increased, and the typical inflammation and fibrosis of CD intestines observed in the MC group. The HPM, MWM and SASP groups were significantly decreased the contents of TNF-α and TGF-β1, and the morphological changes in CD intestines improved in different degree, but the effects of HPM and MWM groups were better than those of SASP group. Conclusion： It has shown that moxibustion therapy can reduce intestinal inflammation and fibrosis in CD, which may be due to down-regulating TNF-α and TGF-β1 in intestinal mucosa and improving intestinal morphology.
出处
《中国中医基础医学杂志》
CAS
CSCD
北大核心
2009年第11期864-867,共4页
JOURNAL OF BASIC CHINESE MEDICINE
基金
国家自然科学基金资助项目(30772831)
国家重点基础研究发展计划(973计划)资助项目(2009CB522900)
