摘要
目的:研究防风色原酮提取物对大鼠胶原诱导性关节炎(collagenⅡ-induced arthritis,CIA)的防治作用及初步机制。方法:Ⅱ型胶原二次免疫诱导大鼠CIA模型,初次免疫前3 d分别以0.21,0.42,0.84 g.kg-1量ig防风色原酮提取物,动态观察大鼠关节炎积分和发病率;免疫后35 d取材,观察关节组织病理学和放射影像学变化;酶联免疫吸附法检测血清抗Ⅱ型胶原抗体IgG和IgM水平;放射免疫分析法检测血清中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)含量。结果:防风色原酮提取物能降低大鼠关节炎积分和发病率,抑制血清中IgG和IgM含量,减轻关节滑膜、软骨和骨的组织病理学变化及X光片的骨破坏程度,降低血清IL-1β,TNF-α和IL-6水平。结论:防风色原酮提取物能控制大鼠CIA病情的发生发展,其机制可能与对促炎细胞因子的有效抑制有关。
Objective： To study the effects and mechanism of chromones from Saposhnikovia divaricata on collagen-induced arthritis（CIA） in rats.Method： Wistar rats were intradermally injected with type II bovine collagen at the base of the tail.Rats were treated daily with oral administration of different doses of chromones from Saposhnikovia divaricata from day 3 before the day of the induction of arthritis to day 35,Clinical symptoms as well as clinical scores and incidence were observed.All rats were sacrificed on day 35 after immunization to observe radiologic and histopathologic changes.Antibody to type II collagen was measured by enzyme-linked immunosorbent assay,the serum levels of pro-inflammatory cytokines tumor necrosis factor-α（TNF-α）,interleukin-1β（IL-1β）,and IL-6 were determined using radioimmunoassay.Result： Treatment with chromones resulted in significant delay of onset of arthritis,as well as significant decrease in arthritis incidence,clinical arthritis severity score,radiographic and histopathological arthritis severity scores,Histopathological examination demonstrated that chromones significantly reduced the inflammatory responses,cartilage damage and bone erosion in the joint tissues.Radiologic changes in the arthritic joints were also significantly reduced in the chromones-treated versus vehicle-treated rats.Chromones inhibited the serum levels of IgG and IgM,TNF-α,IL-1β and IL-6.Conclusion：These results suggest that chromones from Saposhnikovia divaricata contribute to an amelioration of arthritis severity in CIA and the mechanisms of chromones on RA might be in part,related to the inhibited activities of the pro-inflammatory cytokines.
出处
《中国实验方剂学杂志》
CAS
北大核心
2009年第12期52-56,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金资助项目(30672318)
