摘要
用RAPD标记对来自不同产地的24份金莲花种质从分子水平开展遗传多样性研究,为综合评价我国金莲花资源现状及植物资源的合理保护提供参考。结果显示,30条RAPD引物共扩增获得194条清晰可辨条带,其中多态性条带108条,多态性位点百分率为55.67%,金莲花总多样性指数为0.1460,居群内遗传多样性为0.0591,Shannon s多样性指数为0.2248;通过UPGMA进行聚类分析,将24份金莲花种质划分为3个大类,根据总遗传多样性和居群内遗传多样性计算不同居群间遗传分化系数为0.6902,基因流估计平均值为0.2244。研究结果表明,目前我国金莲花种质资源的遗传多样性指数偏低,应该尽快采取相应措施,对不同金莲花资源进行合理保护。
The genetic diversity of Trollius populations from different origins were analyzed by RAPD marker in molecular level,and which will be helpful for population evaluation and for further reasonable protection of Trollius germplasm resources in China. 194 score able bands were amplified in total with 108 bands having polymorphism amplified with 30 RAPD primers in the genome DNA of 24 Trollius populations,and the percentage of polymorphic loci was 55.67%. The general gene diversity index was 0. 1460, and the genetic diversity index within populations was 0. 0591 ,the Shannon＇s information index of all was 0. 2248; Based on the UPGMA cluster analysis method,24 Trollius populations were classified into three main groups,and the coefficient of genetic differentiation was 0. 6902,mean gene flow value was 0. 2244 based on the general genetic diversity and genetic diversity within populations. The analysis resuits showed that the genetic diversity of Trollius populations in China were not abundant,and protection strategies should be carried out on Trollius populations with significant genetic diversity.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2009年第4期535-539,共5页
Journal of Plant Genetic Resources
基金
国家"十五"科技攻关项目(2004BA721A16)
国家"十一五"科技支撑计划项目(2006BAI06A13-03)
