摘要
为揭示小麦(Triticum aestivumL.)生理型雄性不育机理,以化学杂交剂SQ-1为诱导剂,构建了普通小麦西农1376不育和可育生理系,用RT-PCR技术分析了3-磷酸甘油醛脱氢酶(GAPDH)和NFUDP4基因在不同发育时期的不育和可育花药中的表达差异。结果表明,与同期对照相比,GAPDH和NFUDP4基因在不育花药单核期到三核期均下调表达,其中在大量花粉粒败育的单核期,GAPDH下调表达尤为显著。因此,小麦生理型雄性不育花药败育过程中,一方面由于GAPDH基因表达受抑制,使糖酵解受阻导致能量供应不足;另一方面,NFUDP4基因下调表达,可能引起线粒体某一Fe-S族蛋白装配需要的分子骨架减少而使其数量不足,从而可能引起Fe-S族蛋白参与的生化反应减弱,如呼吸链电子传递受阻引起能量供应不足,与小麦生理型雄性不育具有一定关系。
Study was conducted to reveal the mechanism of physiological male sterility （PMS） of Triticum aestivum L., a common wheat variety, Xinong 1376 was used to develop PMS line by spraying SQ-1, a new chemical agents （CHA）, on wheat plants at Feeke＇s 8.5 stage and control lines by spraying same volume water; RT-PCR technique was performed to analyze the expression patterns of three phosphoric acid-glycerol dehydrogenase （GAPDH） and NifU-like domain protein 4 （NFUDP4）genes in sterile and fertile anthers at different development stages. The results indicated that the transcription of GAPDH and NFUDP4 gene were remarkably down-regulated during the period of uni-nuelear to tri-nuclear pollen stage, especially at the uni-nuelear pollen stage when a large proportion of pollen had aborted, the transcript of GAPDH was significantly down-regulated as compared to control. Therefore, it was deduced that on one hand the reaction of glyeolysis weakened due to the decreased transcription of GAPDH gene, the energy was seriously in short supply during the course of pollen development, on the other hand the scaffolds for assembly of some Fe-S clusters were deficient due to decreased transcription of NFUDP4 domain gene, thus Fe-S clusters proteins were not effectively assembled and synthesized, which repressed a variety of biochemical reaction, in which the Fe-S clusters were involved, for example, that electron transfer of respiratory chain was inhibited likely result in insufficient energy production in cell. Hence insufficient energy production might cause male sterility of pollen in some extent.
出处
《核农学报》
CAS
CSCD
北大核心
2009年第6期928-934,共7页
Journal of Nuclear Agricultural Sciences
基金
陕西省"13115"科技创新工程重大科技专项(编号:2007ZDKG-020)
