摘要
目的探讨肿瘤坏死因子-αmRNA(TNF-α mRNA)及转化生长因子-β1mRNA(TGF-β1mRNA)在在子痫前期患者胎盘组织中的表达及临床意义。方法采用RT-PCR技术检测45例正常晚期妊娠妇女及56例子痫前期患者(其中轻度子痫前期21例,重度子痫前期35例)胎盘组织中TNF-α mRNA及TGF-β mRNA的表达水平。结果1.轻、重度子痫前期胎盘组织中TNF-α mRNA表达水平分别为:0.371±0.12、0.892±0.347均明显高于正常妊娠组0.138±0.092(均P<0.01),重度子痫前期组高于轻度子痫前期组(P<0.01)。2.轻、重度子痫前期胎盘组织中TGF-β1mR-NA表达水平分别为:0.752±0.133、1.016±0.346均明显高于正常妊娠组0.384±0.098(均P<0.01),重度子痫前期组高于轻度子痫前期组(P<0.01)。结沦子痫前期胎盘组织中TNF-α mRNA及TGF-β1mRNA表达水平明显升高,并且与子痫前期病情发展密切相关,可作为评价病情严重程度的指标。
Objective： To investigate the placental tumor necrosis factor- a （TNF -α mRNA） and transforming growth factor-β1 （TGF - β1 ） mRNA in normal pregnancies and patients with preeclampsia and to study their clinical significance. Methods： RT - PCR was used to measure levels of placental TNF - α and TGF - β1 mRNA in 45 normal late pregnant women and 56 patients with preeclampsia （ 21 mild preeclampsia, 35 severe preeclampsia） . Results： 1. The levels of TNF - α mRNA of placenta in mild and severe preeclampsia were 0. 371 ± 0. 12, 0. 892 ±0. 347 respectively. There were all obviously significant than normal pregnant group 0. 138 ±0. 092 （ P 〈 0. 01 ） . There was all obviously significant in mild preeclampsia than severe preeclampsia （ P 〈 0. 01 ） . 2. The levels of TGF - β1 of placenta in mild and severe preeclampsia were 0. 752± 0. 133, 1. 016 ± 0. 346 respectively. There were all obviously significant than normal pregnant group 0. 384 ± 0. 098 （ P 〈 0. 01 ） . There was all obviously significant in mild preeclampsia than severe preeclampsia （ P 〈0. 01 ） . Conclusions ： The results suggests that the levels of TNF - α mRNA and TGF - β1 mRNA in placenta are all gradually increased in preeclampsia, which was not changed in pregnant hypertension.
出处
《中国优生与遗传杂志》
2009年第12期20-21,共2页
Chinese Journal of Birth Health & Heredity
