摘要
目的探讨TRAIL、Caspase-3及NF—κB在胆管癌发生、发展及侵袭中的作用。方法运用原位杂交、免疫组织化学法检测TRAIL、Caspase-3及NF—κB在胆管癌中的表达情况。结果TRAIL在胆管癌及癌旁正常胆管组织中的阳性表达率分别为45．2％（19／42）、53．3％（8／15），差异无统计学意义（P〉0．05）；其在胆管癌的不同临床、病理特征之间的表达差异亦无统计学意义（P均〉0．05）。Caspase-3在胆管癌组织中的阳性表达率为30．9％（13／42），明显低于癌旁正常胆管组织中的60．0％（9／15）（P〈0．05）；NF—κB在胆管癌组织中的阳性表达率为61．9％（26／42），明显高于癌旁正常胆管组织中的26．7％（4／15）（P〈0．05）。胆管癌中Caspase-3、NF—κB的表达均与患者的性别、年龄、病变部位无关（P〉0．05），而与是否转移扩散、组织分化程度及生存时间有关（P〈0．05）。此外，胆管癌中TRAIL表达与Caspase-3的表达呈正相关（P〈0．05），NF—κB与Caspase-3呈负相关（P〈0．05），TRAIL与NF—κB无相关性（P〉0．05）。结论TRAIL表达无肿瘤特异性，表达水平的高低并不能完全决定胆管癌转移扩散与否，亦不能完全决定其恶性程度和预后。NF—κB的活化、Caspase-3的失活或缺失可能促进了肿瘤的转移扩散，并导致了不良的预后。NF—κB通过抑制Caspase-3的活性，从而阻断了TRAIL介导的凋亡途径，使得组织逃避凋亡而无限增生，导致了肿瘤的发生、发展及转移扩散。
Objective To investigate the function of TRAIL,Caspase-3 and NF-κB among the occurrence, development and malignant invasion of bile duct carcinoma. Methods The in-situ hybrization technique and immunochemistry method were adopted to detect expression of TRAIL, Caspase-3 and NF-κB. Results The positive rates of TRAIL expression in carcinoma of bile duct and paracancer tissue were 45.2 % （19/42） and 53.3 %（8/15）, respectively. The differences were not significant （P 〉0.05）, and there were no evident difference between expression of TRAIL and every clinic pathology faetor（P 〉0.05）. The positive rates of Caspase-3 expression in carcinoma of bile duct were 30.9 %（13/42）, which were obviously lower than those in paracancer tissue, 60.0 %（9/15）（P 〈0.05）. However, The positive rates of NF-κB expression in carcinoma of bile duct were 61.9 %（26/42）, obviously higher than those in paracancer tissue, 26.7 %（4/15）（P〈0.05）. The positive rates of Caspase-3, NF-κB expression were relation to metastasis and proliferation, differentiation degree of tissue and survival time （P 〈0.05）, but were independent of sex, ages and position of eareinoma（P 〉 0.05）. Moreover, TRAIL and Caspase-3 manifested positive relationship （P 〈0.05）. On the contrary , NF-κB and Caspase-3 manifested negative relationship （P 〈0.05）. However, there was no relationship between TRAIL and NF-κB（P 〉0.05）. Conclusion Expression of TRAIL has no selectivity of tumour; Its expression is higher or lower which couldn＇ t absolutely decide tumour＇s metastasis and proliferation, and could not decide malignant degree and prognosis of tumour, too. Activation of NF-κB and losing action or absence of Caspase- 3 possibly accelerate metastasis and proliferation of tumour, which result in bad prognosis. NF-κB interdicte TRAIL inducing apoptosis approach via control activation of NF-κB, which induce tissue to avoiding apoptosis and multiplication unboundedly, leading to tumour＇s occurren
出处
《肿瘤研究与临床》
CAS
2009年第12期826-829,共4页
Cancer Research and Clinic
