摘要
目的:观察7个月病程的糖尿病大鼠视网膜毛细血管及视网膜神经节细胞的组织病理学改变。方法:链脲佐菌素诱导实验性糖尿病大鼠模型,在模型建立7个月后摘取大鼠眼球,制作视网膜病理切片和视网膜消化铺片,在光镜和透射电镜下观察视网膜毛细血管及视网膜神经节细胞的形态改变。结果:光镜下大鼠视网膜各层组织排列极度紊乱,毛细血管极度扩张,周细胞核固缩,内皮细胞增生,出现无细胞毛细血管、影周细胞及内皮细胞凋亡等糖尿病视网膜病变的特征性改变,视网膜神经纤维层明显水肿。神经节细胞数目减少,胞核固缩,染色质边缘聚集,内外颗粒层排列紊乱。电镜下视网膜毛细血管内皮细胞明显肿胀,胞体变圆,突向管腔,线粒体肿胀,空泡化,基底膜增厚,视网膜神经节细胞胞体变形,核明显固缩,核膜消失,胞浆空泡化,线粒体肿胀,细胞表面突起减少,多聚核糖体及粗面内质网显著减少。结论:糖尿病大鼠在视网膜微血管病变的同时,视网膜神经节细胞也发生损害。
Objective：To observe the pathologic changes of the retinal ganglion cells（RGCs）and capillary in rats with diabetes mellitus for seven months.Methods：Diabetic rats were induced by streptozotocin.After seven months we removed the eyes under anesthesia and made retinal slides and digest preparations.The RGCs and capillary were observed by light and electron microscopy.Results：In light microscopy the retinal tissues were indistinct disturbance and capillaries were enlarged.There were some early symptoms of diabetic retinopathy （DR）,such as extensive pericyte loss,endothelial cell proliferation,pericyte ghost,acellular capillary and apoptosis of endothelial cell.Retinal nerve fiber layer was edematous,nuclear shrinkage and mitochondrion condensed.In electron microscopy endothelial cell of capillary and mitochondrial was edematous obviously,the basement membranes were thickened.RGCs were vacuolated,chromatin of nucleus aggregated marginally decreased and densification.Some of RGCs were necrosis,nucleus were dissolved,mitochondria was edematous,the rough endoplasmic reticula decreased obviously.Conclusion：In the early stage of DR in rats,accompanying the retinal capillary,the RGCs were damaged.
出处
《中日友好医院学报》
2009年第6期350-352,F0002,共4页
Journal of China-Japan Friendship Hospital
关键词
糖尿病视网膜病变
光镜
电镜
大鼠
diabetic retinopathy
light microscopy
electron microscopy
rat
