摘要
目的研究钨酸锂对人淀粉样前体蛋白及早老素-1(M146L)细胞分泌β-淀粉样蛋白(Aβ1-42)的影响及其对体外培养新生小鼠大脑皮层神经元的保护作用。方法①体外培养转染AD相关基因、可分泌Aβ1-42的M146L细胞,在培养液中加入低、中、高剂量(3.1、5、8mmol/L)钨酸锂或阳性对照药,通过对M146L细胞活性及培养基中Aβ1-42含量的测定,研究钨酸锂对M146L细胞分泌Aβ1-42的影响。②体外无血清培养新生小鼠大脑皮层神经元,采用NSE免疫细胞化学染色法进行神经元鉴定;观察神经元突起生长状况,分析研究钨酸锂对大脑皮层神经元的神经保护作用。结果①低、中、高剂量钨酸锂对M146L均无细胞毒性;②低、中、高剂量钨酸锂可抑制M146L细胞分泌Aβ1-42;③原代培养的新生小鼠大脑皮层神经元,经NSE免疫细胞化学染色被染成棕褐色的细胞占绝大多数,表明所培养细胞基本为神经元;④除低浓度(0.008mmol/L)外,终浓度分别为0.2,0.04mmol/L的钨酸锂均能明显使突起数目和长度增加,可促进神经元的存活。结论适量的钨酸锂可抑制M146L细胞分泌Aβ1-42;对新生小鼠大脑皮层神经元具有神经保护作用。
Objective To explore the effects of lithium tungstate（LT） on the secretion of amyloid beta-protein 1-42（Aβ1-42）of M146L and the neuropretection on neurons from cerebral cortex of newborn mouse in vitro.Methods ① M146L cells were culture by low,middle and high dose of LT or positive control drug and the cell activity and the content of Aβ1-42 were detected.② The neurons from cerebral cortex of newborn mouse were cultured in B27-supplemented neurobasal,a new serum-free medium combination,and identified by NSE staining;MTT assay was carried out to investigate the effect of the LT on development of neurons.Results ①Different concentrations of LT had no influence on the survival of M146L cells;② Different concentrations of LT could inhibit the secretion of Aβ1-42 by M146L cells;③Most of the neurons were stained to be positive with NSE,which suggested them being neurons;④The results showed that LT（0.04 mmol/L,or 0.2 mmol/L） could increase the number and the length of neurites as well as the number of survival neurons.Conclusions Adequate LT could inhibit the secretion of Aβ1-42 by M146L cells and has a neuroprotective effect on the neurons from cerebral cortex of newborn mouse.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2009年第22期2877-2880,共4页
Chinese Journal of Gerontology
