摘要
目的制备新型高效的基因工程重组抗原,用于汉滩病毒的快速诊断和分型诊断。方法聚合酶链反应(PCR)扩增得到汉滩病毒S基因的截短片段,将该片段克隆入原核表达载体,并在大肠杆菌中高效表达,将包涵体进行胶上回收,Western blotting分析其抗原性。结果纯化得到相对分子质量约为46×103的重组蛋白,可与肾病综合征出血热(HFRS)阳性血清发生反应,健康人血清为阴性对照。结论研究得到的重组核蛋白在HFRS的血清学诊断中具有一定的应用价值。
Objective To set up highly new efficient recombinant nucleocapsid protein by genetic engineering technique for the rapid diagnosis and analy of the hantann virus. Methods A truncated genetic fragment of Hantaan virus S gene was amplified through PCR technique,and the genetic fragment was cloned into Prokaryotic expression vector and efficiently expressed in the E. coli,and then collected the inclusion bodies through Gel electrophoresis . The antigenicity of the recombinant nucleocapsid protein was verified by Western blotting. Results The molecular of the purified Sh was 46 × 10^3 , The recombinant nucleocapsid protein could react with the HFRS-positive sera, but could not react with the sera of normal healthy persons. Conclusion The prokaryotic expression nucleocapsid protein of the specific hantaan virus have broad prospects in the serodiagnosis of the hemorrhagic fever with renal syndrome （HFRS）.
出处
《检验医学与临床》
CAS
2009年第23期2018-2019,2021,共3页
Laboratory Medicine and Clinic
关键词
汉滩病毒属
重组核蛋白
免疫印迹法
hantaan virus
recombinant nucleocapsid protein
western blotting
