摘要
报道了Ｌ谷酰胺产生菌Ｂｃ－６６诱变育种及其摇瓶发酵条件研究的结果。以黄色短杆菌ＡＴＣＣ１４０６７为出发菌种，经逐级诱变处理，选育１株能高产Ｌ谷酰胺，副产物较少，且对磺胺胍具抗性的变异株Ｂｃ－６６。在适宜的培养条件下，摇瓶发酵７２ｈ，可积累谷酰胺达３１～３３ｍｇ·ｍＬ－１，且遗传稳定性较好。对该菌株的发酵条件作了较系统的试验，摸索出较以前简化的培养基，有利于今后的进一步研究。
The selective breeding of an L glutamine producer and its fermentative conditions in shaking flask were studied.Mutant Bc 66 was derived from Brevibacterium flavum ATCC14067 by means of mutagenesis with diethyl sulphate(DES).The mutant showed a good character.Such as higher product of L glutamine,less by products and good resistance to sulfaguanidine(SG).It can accumulate 31～33 mg·mL -1 L glutamine after 72 hrs in shaking flask culture under optimum conditions.The fermentative medium and conditions were also discussed in this paper.
出处
《江西科学》
1998年第4期242-248,共7页
Jiangxi Science
关键词
谷酰胺
黄色短杆菌
育种
发酵
菌株
L glutamine, Brevibacterium flavum ,Breeding,Fermentation
