摘要
目的建立了RP-HPLC法测定人血浆中头孢妥仑的浓度。方法色谱柱Nucleodur C18分析柱(4.6mm×250mm5μm),流动相为0.03%三氟乙酸缓冲液/乙腈(81/19,V/V),流速1.0ml/min,检测波长为305nm,柱温30℃,取上清液直接进样,进样量为20μL结果头孢妥仑线性范围为0.02～5.0μg/mL。头孢妥仑的最低检测限为0.02μg/mL,日内、日间RSD均小于5%,相对回收率为97.6%～104.5%,提取回收率均大于91.3%。结论这种验证方法灵敏、简便、可重复,足以用于药代动力学研究。
Objective To establish an HPLC method for determination of cefditoren in plasmal. Methods Nucleodur C18 was used with the mobile phase of 0. 03 % trifluoro acetic acid buffer/acetonitrile （81/ 19, v/v） at the detection wavelength of 305nm. The flow rate was 1.0 ml/min. Results Under the condition of this cefditoren in plasma was well separated, the calibration curve was linear in the range of 0. 02 - 5.0 μg/ml. The limit of quantification was 0. 02μg/ml. The RSDs of intra- and inter-day were less than 5%, the rela- tive recovery was 97. 6% - 104. 5% and the extraction recovery was above 91.3%. Conclusion This validated method is sensitive, simple and repeatable enough to be used in pharmacokinetic studies.
出处
《中国实用医药》
2009年第35期12-13,共2页
China Practical Medicine
