摘要
目的 探讨反复吸入七氟醚对老龄大鼠海马组织凋亡蛋白酶活化因子-1（Apaf-1）及半胱氨酸天冬氨酸蛋白酶-9（Caspase-9）mRNA表达的影响。方法健康Wistar大鼠30只，月龄22月，体重380～770g，雌雄各半，随机分为4组：对照组（n=8，Ⅰ组）、2％七氟醚组（n=8，Ⅱ组）、3％七氟醚组（n=8，Ⅲ组）及颈总动脉结扎＋2％七氟醚组（n=6，Ⅳ组）。Ⅰ组不吸入七氟醚，Ⅱ组及Ⅲ组分别吸入2％、3％七氟醚，100min／d，连续5d。Ⅳ组结扎左侧颈总动脉，1周后吸入2％七氟醚，100mirgd，连续5d。停止吸入七氟醚后第1天开始采用Morris水迷宫实验测定大鼠认知功能，4次／d，连续测定5d（T1-5），记录潜伏期、平台象限活动时间与总游泳时间的百分比（Tp/T）及撤去平台后2min内的游泳轨迹。水迷宫实验结束后采用RT-PCR法测定海马组织Apaf-1、Caspase-9mRNA的表达水平。结果与T1时比较，Ⅰ组L3-5、Ⅱ组及Ⅲ组T2-5时、Ⅳ组L4.5时潜伏期缩短（P〈0．05或0．01）；与Ⅰ组比较，Ⅲ组T1时、Ⅳ组L1-5时潜伏期延长，Ⅲ组及Ⅳ组Tp／T降低，Ⅳ组Caspase-9mRNA表达上调（P〈0．05或0．01）。撤去平台后，Ⅱ组大鼠游泳轨迹明显集中在平台象限（Ⅳ象限），Ⅲ组和Ⅳ组大鼠游泳轨迹明显散乱。结论反复吸入七氟醚可导致老龄大鼠认知功能障碍，其机制与海马组织Apaf-1及Caspase-9 mRNA的表达无关。
Objective To investigate the effects of repeated inhalation of sevoflurane on the expression of apoptotic protease-activating factor-1 （Apaf-1） and Caspase-9 mRNA in hippocampus in elderly rats. Methods Thirty Wistar rats （ 15 males, 15 females） aged 22 months weighing 380-770 g were randomly divided into 4 groups： control group （ n = 8, group Ⅰ ）, 2% sevoflurane （ n = 8, group Ⅱ ）, 3% sevoflurane （ n = 8, group Ⅲ ） and ligation of left common carotid artery ＋ 2 % sevoflurane group （ n = 6, group Ⅳ ）. Group Ⅰ inhaled oxygen （2.5 L/min） and nitrogen （6 L/min）. Group Ⅱ and Ⅲ inhaled 2% and 3% sevoflurane in oxygen （2.5 L/rain） and nitrogen （6 L/min） 100 min/d for 5 consecutive days respectively. In group Ⅳ, left common carotid artery was ligated and 2% sevoflurane inhaled 100 min/d for 5 consecutive days one week later. Cognitive function was detected 4 times a day for 5 consecutive days using Morris water-maze （MWM） test after the last inhalation （T1-5 ）. The latency, percentage of swimming time in the platform quadrant （Tp/T） and swimming traces within 2 min after the removal of platform were recorded. The rats were sacrificed immediately after the last test and hippecampus immediately removed for determination of Apaf-1 and caspase-9 mRNA expression using RT-PCR. Results The latency at T3-5 in group Ⅰ, T2-5 in group Ⅱ and Ⅲ and T4.5 in group Ⅳ was significantly shorter than that at T1 （ P 〈 0.05 or 0.01 ）. The latency was significantly prolonged at T1 in group Ⅲ and at T1-5 in group Ⅳ , Tp/T significantly decreased in group Ⅲ and Ⅳ and Caspase-9 mRNA expression up-regnlated in group Ⅳ compared with group Ⅰ （P 〈 0.05 or 0.01 ）. After the platform was removed, the swimming traces were located in platform quadrant （quadrant Ⅳ ） in group Ⅱ , while obviously in disorder in group Ⅲ and Ⅳ . Conclusion Repeated inhalation of sevoflurane can result in cognitive dysfunction and the mechanism is not related t
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2009年第10期903-907,共5页
Chinese Journal of Anesthesiology
