摘要
目的探讨褪黑素(melatonin,MT)对马桑内酯致痫大鼠海马内P物质水平的影响,以探讨褪黑素的抑痫作用机制。方法随机将健康成年雄性SD大鼠40只分为A、B、C、D 4组,每组10只。A组:生理盐水组;B组:马桑内酯组;C组:褪黑素+马桑内酯组;D组:Luzindole+褪黑素+马桑内酯组。观察并记录行为学变化,然后分别采用免疫组织化学方法进行SP免疫组织化学染色,实时荧光定量PCR方法检测海马内SP mRNA含量变化。结果B组和D组大鼠均有不同程度的癫痫发作,而C组大鼠癫痫发作不明显,A组几乎均无发作;免疫组化结果显示,四组大鼠海马各区均有SP免疫反应阳性神经元,B组、D组与A组、C组比海马内SP免疫阳性反应明显增强(P<0.05),而A组与C组比无明显差异(P>0.05);RT-PCR结果提示,B组、D组与A组、C组相比,大鼠海马内SP mRNA明显升高(P<0.05),而A组与C组比无明显差异(P>0.05)。结论MT能通过下调海马内SP水平抑制癫痫发作。
Objective To investigate the mechanism underlying the effects of melatonin（MT） on seizure induced by coriaria lactone,by studying its effect on substance P levels in rat hippocampus.MethodsForty healthy adult male SD rats were randomly divided into groups A,B,C and D of 10 rats each.Rats of group A were given intracerebroventricularly（i.c.v.）normal saline;rats of group B were injected with Coriaria lactone;Rats of group C were injected with MT intraperitoneally（i.P.） and Coriaria lactone;rats of group D were given luzindole（i.c.v.）,MT（i.P.）and Coriaria lactone（i.c.v.）.The behavior of the animals was observed and recorded,the change of SP protein and mRNA in the hippocampus was detected by immunohistochemistry or RT-PCR.ResultsThe animals in groups B and D showed epileptic convulsions,electric discharge,and varying degrees of seizures,while the other groups did not.The immunohistochemical staining showed SP positive immunoreaetion in all four groups.It was obviously stronger in groups B and D than in groups A and C（P〈0.05）,but,there was no significant difference between group A and group C（P〉0.05）.RT-PCR revealed that SP mRNA in hippocampus of groups B and group D was significantly higher than that of groups A and C（P〈0.05）,with no significant difference between group A and group C（P〉0.05）.ConclusionMT may prevent the seizure induced by Coriaria lactone in rats by down-regulating the expression of substance P in rat hippocampus.
出处
《中国组织化学与细胞化学杂志》
CAS
CSCD
2009年第6期638-644,共7页
Chinese Journal of Histochemistry and Cytochemistry
基金
华中科技大学科学研究基金(189135007)
关键词
癫痫
褪黑素
马桑内酯
P物质
Epilepsy
Melatonin
Coriaria lactone
Substance P
