摘要
为探讨乳腺癌患者外周血单个核细胞(PBMC)介导LAK样杀伤作用和ADCC(抗体依赖性细胞介导的细胞毒)作用下降的机制,观察IL-2增强乳腺癌患者PBMC介导杀伤作用的效果。应用MTT法检测LAK样杀伤作用;应用非核素细胞毒试剂盒(LDH释放法)检测ADCC杀伤作用;应用流式细胞术检测CD3+CD8+T细胞、CD3-CD56+NK细胞水平和CD16ζ的表达。结果显示:进展期乳腺癌患者PBMC中CD3+CD8+T细胞、CD3-CD56+NK细胞及其CD16ζ的表达水平与健康对照组相比明显降低,有统计学意义(P<0.01)。进展期乳腺癌患者PBMC经IL-2(1 000 U/ml)处理5 d后,CD3+CD8+T细胞和CD3-CD56+NK细胞比例明显增加,CD16ζ的表达水平明显提高。在各效靶比时,IL-2处理后进展期乳腺癌患者PBMC对SKBR3细胞的LAK样和ADCC杀伤率明显高于未处理组,差异有统计学意义(P<0.01);提示进展期乳腺癌患者PBMC介导的LAK样杀伤活性和ADCC作用明显削弱,CD3+CD8+T细胞、CD3-CD56+NK细胞比例和CD16ζ的表达水平明显降低,IL-2可以刺激CD3+CD8+T细胞和CD3-CD56+NK细胞增殖活化,促进CD16ζ的表达,增强杀伤作用。
To explore the mechanism of reduction LAK cytotoxieity and ADCC mediated by PBMC from advanced cases of patients with breast cancer and the enhancement of anti-tumor cytoxicity induced by IL-2, MTT assay was used to investigate the eytotoxicity and the LDH-release assay was used to test ADCC. Meanwhile, flow cytometry assay was employed to detect the expression of CD3^＋ CD8^＋ T cells, CD3^＋ CD56^＋ NK cells and CD16ξ. It was found that the levels of CD3^＋ CD8^＋ T cells and CD8^＋ CD56^＋ NK cells in PBMC from advanced cases of patients with breast cancer was down regulated, while the expression level of CD16ξ in NK cells was significantly reduced compared with that in early disease or healthy individuals. The CD3^＋ CD8^＋ T cells and CD3^＋ CD56^＋ NK cells in PBMC from advanced breast cancer patients treated by IL-2 as well as the expression level of CD16ξ in NK cells was significantly up-regulated, and the cytotoxicity of PBMC treated by IL-2 against SKBR3 cell was also significantly improved. It is evident that the function of LAK cytotoxicity and ADCC may be impaired in PBMC from advanced breast cancer patients in comparison with that in early eases of patients orhealthy individuals. In the advanced cases of patients, the expression levels of CD3^＋ CD8^＋ T cells, CD3 ^＋CD56^＋ NK cells and CD16^＋ are significantly down-regulated, but IL-2 can enhance the proliferative activity of CD3^＋ CD8^＋T cells, CD3^＋ CD56^＋ NK cells and the expression levels of CD16ξ, thus improving their eytotoxicity effect.
出处
《现代免疫学》
CAS
CSCD
北大核心
2009年第6期505-509,共5页
Current Immunology
