摘要
[目的]利用RAPD标记分析葱属7个栽培品种的遗传多样性。[方法]采取改良CTAB法提取植物的总DNA,从68个RAPD引物中筛选出25条具有多态性且扩增稳定的引物用于品种的DNA多态性分析,采用优化后的RAPD的PCR反应体系进行PCR扩增,利用MEGA4软件进行各品种间UPGMA聚类分析。[结果]从25个引物中共扩增出245条DNA带,其中99条为多态性带。根据聚类分析,葱属7个栽培品种可分为2类:汉中冬韭、阜丰1号和豫韭2号为1类;其他4个为1类,其中怀化大蒜和南宁大蒜、连葱6号和杭州分葱各为1小类;7个品种的相似系数范围为80.2%～99.8%。该聚类结果与依据表型特征的传统分类的结果一致。[结论]该研究为准确地进行葱属植物种质资源的鉴定、筛选和利用提供了科学依据。
[ Objective ] The purpose was to analyze the genetic diversity of 7 cuhivars of Allium based on RAPD markers. [ Method ] The total DNA in plant was extracted by improved CTAB method and 25 RAPD primers with polymorphism and stable amplification were selected from 68 RAPD primers to analyze DNA polymorphism of 7 cuhivar of Allium. PCR amplification was made by PCR reaction system of optimized RAPD. The UPGMA cluster analysis on various varieties was conducted by MEGA4 software. [ Result ] 245 DNA bands were amplified out from 25 primers,among which, 99 were polymorphism bands. According to cluster analysis, 7 cuhivars of AUium were divided to 2 classes： A. tuberosum cv. Hangzhong Winter Leek, A. tuberosum cv. Fufeng No. 1 leek and A. tuberosum cv. Henan No. 2 leek belonged to a class ; the others belonged to a class including 2 branches, in which, A. sativum cv. Huaihua garlic and A. sativum cv Nanning garlic belonged to a branch ; A. cepa cv. Liancong No. 6 onion and A. fistulosum var. caepitusum cv. Hangzhou Spring shallot belonged to the other branch. The similarity coefficients in 7 cuhivars of Allium were from 80.2% to 99.8%. The result of cluster analysis was consistent with the traditional classification based on morphologic traits. [ Conclusion] This study provided the scientific basis for accurate identification, screening and utilization of Allium plant germplasm resource.
出处
《安徽农业科学》
CAS
北大核心
2009年第35期17382-17383,17385,共3页
Journal of Anhui Agricultural Sciences
基金
怀化学院民族药用植物资源研究与利用湖南省重点实验室资助项目
