摘要
目的探索重组结核分枝杆菌Rv1009蛋白(rRv1009)在结核分枝杆菌快速培养方面的应用价值。方法以Middlebrook7H9、罗氏培养基为基础培养基,观察rRv1009对卡介苗生长的影响。结果含rRv1009的罗氏培养基于接种卡介苗后第8天出现菌落,比普通罗氏培养基早8～9d;在添加rRv1009的Middlebrook7H9培养基中,卡介苗生长状况明显好于普通Middlebrook7H9培养基。结论rRv1009能促进BCG的复苏和生长,为结核分枝杆菌的快速培养的研究打下了基础。
[ Objective] To explore the effect of the recombined Rv1009 （rRvl009） protein in the rapid culture of Mycobacterium tuberculosis. [Methods] The Middlebrook 7H9 and Lowenstein-Jensen medium（L-J） were used as the culture medium to detect the effect of the rRv1009 in rapid culture of Mycobacterium boris BCG. [Results] The BCG started growing after 8 days of incubation in the L-J with rRv1009, 9 days earlier than the L-J without rRv1009; the Mycobacterium boris BCG growth much better in the Middlebrook 7H9 with rRv1009 than the common ones. [Conclusion] The rRv1009 can promote the resuscitation and growth of Myeobacterium bovis BCG, and the results would constitute the basis for the future studies of rapid culture of MTB.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第22期3410-3413,共4页
China Journal of Modern Medicine
基金
国家自然科学基金资助项目(No:30471648)
