摘要
目的 探讨非小细胞肺癌组织 (NSCLC)中 3p2 5位点上等位基因的杂合性丢失 (LOH)与肺癌发生、发展的相关性。方法 采用银染聚合酶链反应结合二核苷酸 (CA)n重复程序出现多态性评价LOH ,分析 1 58例NSCLC癌组织中 3p2 5等位基因的LOH。结果 1 58例NSCLC组织中 80例出现 3p2 5LOH ,总的杂合性丢失率为 50 .6%。腺癌组织中 3p2 5杂合性丢失率为 62 .0 % ,鳞癌为 43 .2 % ,两者间差异有显著性 (P <0 .0 5)。 3p2 5LOH与NSCLC临床分期关系不明显 ,Ⅰ、Ⅱ及Ⅲ期分别为 52 .2 %、45.0 %及 52 .8% ,4例良性肺肿瘤和 5例正常胎儿肺组织均未出现 3p2 5LOH。结论 本研究结果表明 3p2 5LOH在NSCLC中普遍存在 ,从而说明 3p2 5位点处可能存在着某些与肺癌发生。
Objective To ascertain whether the loss of heterozygosity (LOH) of allele on chromosome 3p25 region is associated with non small cell lung cancer (NSCLC). Methods 3p25 LOH was analysed in patients with NSCLC by polymerase chain reaction based assays for dinucleatide repeat polymorphisms. Results Eighty out of 158(50.6%) cancer tissues of NSCLC were found to have 3p25 LOH, but no 3p25 LOH was observed in 5 normal fetal lung tissues and 4 tissues of the benign lung neoplasms. The deletion was detected in 62.0% of adenocarcinoma and 43.2% of the squamous carcinoma, indicating that the former was significantly higher than the latter (P<0.05) . Loss frequences for TNM stages were further observed in 52.2%, 45.0% and 52.8% of the cases for stage Ⅰ,Ⅱ and Ⅲ respectively, and these data showed no significant relationship between the loss frequence and TNM staging of NSCLC (P>0.05). Conclusion Above findings display that 3p25 LOH appear to be a common structural alteration occurred in NSCLC and there may be suppressor genes relevant to the carcinogenesis of the lung in 3p25 region.
基金
上海市医学领先学科和上海市科委课题
关键词
非小细胞肺癌
3p25
杂合性丢失
PCR
肺癌
Non small cell lung cancer 3p25 Allele Loss of heterozygosity PCR-LOH analysis
