摘要
目的:建立超高效液相色谱-串联质谱(UPLC-MS/MS)测定人血浆中盐酸尼卡地平浓度的方法。方法:血浆经甲基叔丁基醚萃取后,以UPLC分离,电喷雾离子化(ESI+)串联质谱检测。盐酸尼卡地平、尼莫地平(内标)的多反应监测(MRM)扫描离子对m/z分别为480.3→314.8和419.2→343.0。结果:盐酸尼卡地平在0.1～40.0 ng.mL-1范围内线性关系良好(r=0.9998);日内、日间RSD均小于12.1%。结论:该方法检测快速、专一、灵敏,可满足盐酸尼卡地平临床药动学研究的要求。
Objective： To establish an UPLC-MS/MS method for the determination of nicardipine hydrochloride in human plasma. Methods： Nicardipine hydrochloride and internal standard were extracted from plasma with methyl tert-butyl ether and determinated by UPLC-MS/MS using electrospray ionization （ESI-）. The drugs were detected on multiple reaction monitoring mode by the transitions precursor to the product ion （m/z 480.3→314.8 and m/z 419.2→343.0）. Results： Calibration curve was linear in the range from 0.1-40.0 ng·mL^-1 with a correlation coefficient of 0.9998. The intra- and inter-day precision （RSD） were all less than 12.1%. Conclusion： The UPLC-MS/MS method for the quantitative determination of nicardipine hydrochloride in human plasma presented simple process, sensitive detection and acceptable precision. Therefore, it can be used for clinical pharmacokinetic studies of nicardipine hydrochloride in human plasma.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第11期1837-1841,共5页
Chinese Journal of Pharmaceutical Analysis
