摘要
目的:建立反相高效液相色谱法同时测定血浆中奥卡西平(OXC)及其活性代谢产物-10-单羟基代谢物(MHD)的浓度。方法:采用Waters X-TerraTM RP_(18)柱(150mm×3.9mm,5μm),柱温35℃,流速1.2ml·ml^(-1),流动相:甲醇-O.1%三氟乙酸(30:70),检测波长232nm。甲喹酮作为内标,血浆样品经蛋白沉淀法处理后直接进样测定。结果:OXC在0.625～37.50μg·ml^1,MHD在1.25～75.0μg·ml^(-1)的范围线性关系良好。OXC低、中、高三种浓度的提取回收率分别为109.9%,102.0%,104.1%。日内RSD为1.69%～3.61%,日间RSD为2.73%～7.45%;MHD低、中、高三种浓度的提取回收率分别为108.0%,107.3%,102.6%,日内RSD4.41%～5.75%,日间RSD 1.32%～7.06%。结论:该方法准确,简单,快速,可用于OXC临床治疗的药物浓度监测和药物动力学的研究。
Objective ： To establish a HPLC method for the simultaneous determination of oxcarbazepine （OXC） and active metabolite（ 10-monohydroxy metabolite, MHD） in human plasma. Method： The Waters X-TerraTM RP18（ 150mm ×3.9mm, 5μm）was used , flow-rate was 1.2ml. min^-1 at 35 ℃, mobile phase consisted of methanol -0.1% trifuroacetic acid （30： 70）, detection wavelength was 232 nm. Methaqualon was used as internal standard. The samples were extracted by protein precipitation. Result： The method was found to be linear over the concentration ranges （ OXC 0. 625 - 37.50μg.ml^-1 and MHD 1.25 - 75.0μg.ml^-1 ）. The extraction recovery of low, middle and high concentration OXC was 109.9%, 102.0% and 104. 1% respectively, the intra-RSD was 1.69% - 3.61%, and inter-RSD was 2.73% -7.45%. The extraction recovery of low, middle and high concentration MHD was 108.0%, 107.3% and 102.6% respectively, the intra-RSD was 4.41% -5.75%, and inter-RSD was 1.32% - 7.06%. Conclusion： The method is acurate, simple and rapid. It is suitable for the therapeutic drug monitoring and pharmacokinetic research.
出处
《中国药师》
CAS
2009年第12期1689-1691,共3页
China Pharmacist
基金
深圳市科技计划项目(200702144)
