摘要
目的探讨GA（藤黄酸）对膀胱癌细胞株BIU-87生长的抑制作用及其机制。方法分别以倒置相差显微镜、噻唑蓝（MTT）比色法、流式细胞术观察GA对BIU-87的形态学改变作用、生长抑制效应、凋亡诱导以及周期阻滞作用。结果倒置相差显微镜观察发现，GA作用后BIU-87细胞变圆、脱壁，部分死亡、漂浮；MTT法显示，GA对BIU-87具有明显的生长抑制作用，并随着药物浓度的增加（0—3μmol／L）而逐渐增强，IC50为1．7μmol／L；流式细胞术检测发现，GA浓度为1．5、3．0μmol／L时凋亡率分别为（34．6±5．2）％和（58．6±6．9）％，显著高于对照组的（2．2±0．8）％；GA浓度为0、1．5、3．0μmol／L，作用48h后，G0～G1期细胞数分别是（35．0±1．2）％、（35．0±1．5）％、（46．0±3．1）％；而S期细胞比例分别为（33．5±0．7）％、（34．1±0．6）％、（15．6±3．3）％，并呈剂量依赖性阻滞BIU-87细胞周期于G。／G，期。结论GA对BIU-87细胞有明显生长抑制作用，这可能与诱导BIU-87凋亡、阻滞其细胞周期进程有关。
Objective This study was purposed to explore the inhibition effect and the mechanism of gambogic acid（GA） on BIU-87cells. Methods Cell morphological assessment and MTT chromometry were both used to observe the growth inhibition of BIU-87 cells treated by gambogic acid ,flow cytometry was also used to detect the apotposis and the cell cycle. Results Under inverted-microscope,typical morphological changes of growth inhibition were observed, MTT assay indicated that BIU-87 cells were significantly inhibited in growth in a dose-dependent manner and the 48 h IC50 was 1.7 μmol/L. Flow cytometric assay showed that the apoptotic rates also was enhanced along with increasing of drug concentration, and the apoptotic rates resulting from 0,1.5,3.0μmol/L GA were （ 2.2 ± 0.8） %, （ 34.6 ± 5.2） % and （58.6±6.9）% respectively. Mter 48 h with different concentration GA cell （0,1.5,3.0 μmol/L）, G0-G1 stage cell numbers were （35.0 ± 1.2） %, （35.0 ± 1.5 ） %, （46.0± 3.1 ） %, and S stage cell numbers were （33.5 ±0.7）%, （34.1 ±0.6）%, （15.6± 3.3）%. The cycle of BIU-87 was arrested in G0/ G1 phase. Conclusion GA could significantly inhibit the growth of BIU-87 celts,possibly through the induction of apoptosis and the arrest of cell cycle.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2009年第12期1676-1677,共2页
Chinese Journal of Experimental Surgery
