摘要
目的研究不同病理类型人类结直肠肿瘤中MTS1基因产物p16蛋白的表达情况;探讨其异常表达与肿瘤发生发展的关系。方法采用免疫组织化学方法,对136例结直肠肿瘤石蜡标本的p16蛋白进行测定。结果 p16蛋白在结直肠癌中的阳性检出率为83.1%,明显高于切缘粘膜的1.2%和非肿瘤患者结肠粘膜的10%(P<0.01)。管状腺癌的阳性率为94.1%,显著高于绒毛状腺瘤恶变、乳头状腺癌和粘液腺癌(P<0.05)。管状腺癌的阳性表达强度显著高于乳头状腺癌(P<0.01)和绒毛状腺瘤恶变(P<0.05)。结论在结直肠肿瘤中,MTS1基因有高频率的异常表达。这种异常表达在各种病理类型的大肠肿瘤都是常发现象。该基因在各种病理类型的结直肠肿瘤中都有着高频率的基因变异,与结直肠肿瘤的发生与发展密切相关。
Objective To study the expression of p16 protein, the product of MTS1 gene, in different patho-logical types of human coloreetal tumors, and investigate the relationship between the abnormal expres-sion with colorectal neoplasm generation and progression. Methods The expression of p16 protein in 136 cases of paraffin-embedded specimens of human colorectal tumors was examined by immunohis-tochemical method. Results p16 protein positive rate of 83.1% in colorectal neoplasm was markedly higher than 1.2% in verge mueosa of excised tissues and 10% in non-tumor patient's colonic mucosa (P<0. 01). The positive rate of 94. 1% in tubiformadenocarcinoma was significantly higher than in can-ceration of villiform adenoma ,papilloadenocarcinoma and myxo-adenocarcimoma (P<0. 05). The intensi-ty of expression in tubiforadenoeareinoma was obviously higher than in papillo-adenocareinoma (P<0. 01) and in canceration of villiform adenoma (P<0. 05). Conclusion In colorectal neoplasm, MTS1 gene has an abnormally high frequency expression which is a common event in all kinds of human col-orectal tumors, suggesting that the gene alteration with a high frequency in various pathological types of colorectal tumors would be closely related with generation and progression of tumors.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
1998年第6期499-500,共2页
Chinese Journal of Experimental Surgery
关键词
直肠肿瘤
结肠肿瘤
MTS1基因
免疫组织化学
Colorectal neoplasm Multiple tumor suppressor MTS1 gene, Immunohistochemical method
