摘要
目的：通过观察血管紧张素-（I-7）（Ang-（1-7））作用后糖尿病大鼠肾脏PDGF（血小板源性生长因子）、TGF-β1（转化生长因子B1）、CollagenⅣ（Ⅳ型胶原）的变化，研究其对糖尿病大鼠肾脏的作用。方法：将SD大鼠分为单肾切除对照组（C组）、糖尿病模型Ang-（1—7）干预组（T组）及糖尿病模型对照组（D组）三组进行研究。分别检测24h尿总蛋白等；同时光镜下观察肾脏组织改变情况并检测肾脏组织内PDGF、TGF-β1、CollagenⅣ基因表达水平。结果：T组大鼠肾重体重比（RW／BW）、尿蛋白定量、mRNA表达水平等指标明显下降，与D组相比具有显著性差异（P〈0．01），肾脏组织光镜下差别显著。结论：Ang-（1-7）具有延缓糖尿病大鼠肾脏病变发生、发展的作用。
Aim To study the effect of angiotensin - （ 1 - 7） on the kidney of diabetic rats by observing the mRNA expression of PDGF, TGF - β1 and Col- lagen Ⅳ . Methods SD rats were divided into three groups as following： Group C （uni -nephrectomy control group） , Group D （diabetic modal control group）, Group T （ Ang- （1 -7 ） treated group）. We evaluated urine albumin excretion etc. , study the renal morphology by light microscope ,and detect the gane expression of PDGF, TGF - β1 and CoLlagen Ⅳ in renal tissue. Results there is significant difference between the group D and T about the RW/BW,Renal Morphology, the total urine protein and the mRNA expression of PDGF, TGF-β1 and Collagen Ⅳ . Conlusion Ang - （ 1 - 7 ） can relieve the process in the kidney of diabetic rats.
