摘要
目的:探讨人早孕母胎界面表达TSLP及其受体(TSLPR)的特征。方法:使用RT-PCR、Western blot、免疫组织化学法分析正常绒毛组织TSLP/TSLPR的表达;RT-PCR、免疫细胞化学法分析原代培养的滋养细胞TSLP/TSLPR的表达;ELISA检测原代培养的人滋养细胞上清TSLP分泌水平。结果:从转录水平至蛋白水平,绒毛细胞滋养细胞与合体滋养细胞均表达TSLP及TSLPR。结论:TSLP表达于正常早孕绒毛组织,可能与早孕期调节性T细胞的扩增及功能变化相关。
Objective：To investigate the expression of a new cytokine, thymic stromal lymphopoietin （TSLP） and its receptor TSLPR in the villi of human first trimester gestation. Methods： Villi were collected from women who had undergone an artificial abortion at 7-11 weeks of normal gestation. The trophoblast cells （Tros） were isolated and cultured. The total RNA was extracted using TRIzol reagent, from both villi and the Pereoll-gradient-isolated Tins,then the DNA fragments of hTSLP and hTSLPR were amplificated by RT-PCR. Villous tissues were detected for TSLP by immtmohistoehemistry （IHC） and Western blot. Immunoeytoehemistry （ICC） was carried out on cultured trophoblast cells for TSLP/TSLPR expression. Levels of TSLP in the supernatants were detected by ELISA. Results： Normal villi and the cultured Tros transcript were found to express TSLP/TSLPR mPNA and secreted TSLP protein. In addition, TSLP receptor was also expressed on trophoblasts. Conclusion： Both TSLP and its receptor are expressed on villi and trophoblast cells, which suggests that TSLP plays an important role in maternal-fetal immuno-toleranee in human early pregnancy.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2009年第11期1019-1022,共4页
Chinese Journal of Immunology
基金
国家重大科学研究计划项目(2006CB944007)
国家自然科学基金重点项目(30730087)
国家自然科学基金面上项目(30872768)
上海市重点学科建设项目(B117)
上海市医学领军人才基金项目资助
关键词
胸腺基质淋巴细胞生成素
母胎界面
调节性T细胞
Thymic Stromal Lymphopoietin（TSLP）
Maternal-fetal interface
T regulatory cell（Treg）
