摘要
目的研究扩增受阻突变体系-聚合酶链反应（ARMS—PCR）和限制性酶切片段多态性-聚合酶链反应（RFLP—PCR）技术的优缺点，探讨其在单核苷酸多态性（SNP）检测及分析中的应用。方法采用ARMS—PCR和RFLP—PCR技术检测分析山西省子宫颈癌患者及健康对照组MMP-2基因启动子区域-1306C／T多态性，其结果经DNA测序验证。结果经ARMS—PCR扩增出MMP-2基因启动子CC、CT、TT三种基因型相应的DNA片段，与DNA测序结果符合。结论ARMS—PCR技术操作简便、省时、成本低，结果稳定可靠，对于一些SNP的研究具有较高的应用价值。
Objective To compare the advantages and disadvantages of the amplification refractory mutation system-polymerase chain reaction （ARMS-PCR） with restriction fragment length polymorphism- polymerase chain reaction（RFLP-PCR） technology, then explore its application in detection and analysis of single nueleotide polymorphism （SNP）. Methods By using ARMS-PCR and RFLP-PCR the polymorphisms of the MMP-2 in cervical cancer and the normal-control group were analyzed. The research sites were-1306 C/T. The final result was verified by DNA sequencing. Results The results of amplified used ARMS-PCR showed that the genotypes of the SNP site in MMP-2 gene promoter were CC, CT, TT, and the DNA fragments were corresponding with the results of DNA sequencing. Conclusion ARMS-PCR technology has the advantages of lower cost time-saving simple operation and reliable results, while has a high value for research of SNP.
出处
《肿瘤研究与临床》
CAS
2009年第11期742-744,共3页
Cancer Research and Clinic
基金
山西省自然科学基金（2006011121）
关键词
子宫颈肿瘤
聚合酶链反应
多态性
单核苷酸
MMP-2
Uterine cervical neoplasms
Polymerase chain reaction
Polymorphism, single nucleotide
MMP-2
