摘要
以生长旺盛的地锦嫩茎为材料,进行了愈伤组织的诱导和分化、不定芽的分化及试管苗的生根、移栽、扦插和移植的研究,结果证明:MS+BA 0.4～0.6mg/L+2,4-D 1.5～2.0mg/L是诱导嫩茎形成具有分化能力愈伤组织的理想培养基;MS+AgNO3 0.8mg/L+BA 0.6mg/L+NAA 0.1mg/L是诱导愈伤组织分化培养的理想培养基;B5+BA 0.5mg/L+NAA 0.1mg/L是不定芽分化培养的理想培养基;B5+IAA0.2mg/L+NAA 0.1mg/L是试管苗生根继代培养的理想培养基;移植的试管苗具有生长旺盛整齐、根系发达、开花结果时间推迟15d左右的特点。
Euphorbia humifusa was used as materials in order to make research on callus induction,adventitious buds differentiation and taking roots,transplanting and cutting of test-tube plantlets. The results indicated that the optimum medium for tender stem inducing and differentiating to callus was MS＋BA 0.4-0.6mg/L＋2,4-D 1.5-2.0mg/L ,the optimum medium for inducing and differentiating callus was MS＋ AgYO3 0.8mg/L＋BA 0.6mg/L＋NAA 0.1mg/L ;B5＋BA 0.5mg/L＋NAA 0.1mg/L was ideal medium for adventitious buds differentiation ,B5＋IAA 0.2mg/L＋NAA 0.1mg/L was ideal medium for adventitious buds taking roots. The florescence could be lengthened 15 days with the vigorous test-tube plantlets and the flourishing roots.
出处
《现代农业科技》
2009年第21期77-78,81,共3页
Modern Agricultural Science and Technology
基金
辽宁师范大学教学改革项目(LSJG:20090108)
关键词
地锦
愈伤组织
不定芽
培养基
Euphorbia humifusa
callus
adventitious bud
medium
