摘要
目的:采用X染色体连锁凋亡抑制蛋白(XIAP)对缺氧-复氧诱导心肌细胞凋亡模型进行干预,以探讨脂质体介导XIAP基因转染对缺氧-复氧诱导的新生大鼠心肌细胞凋亡的影响。方法:体外培养新生大鼠心肌细胞,分为4组,①XIAP组:将pDsRed2-XIAP以脂质体转染到心肌细胞48h后进行缺氧2h-复氧1h;②预处理组:先将心肌细胞进行缺氧预处理,然后进行缺氧2h-复氧1h;③单纯缺氧-复氧组:将心肌细胞直接进行缺氧2h-复氧1h;④常氧组:将心肌细胞在CO2孵箱中培养3h。各组最后用流式细胞仪Annexin V FITC检测心肌细胞凋亡率结果组间差异。结果:①pDsRed2-XIAP可以通过脂质体转染的方式转染到心肌细胞;②与单纯缺氧-复氧组比较,XIAP组和预处理组心肌细胞凋亡率明显减低,P<0.01;③XIAP组与预处理组比较二者心肌细胞凋亡率相似,P>0.05。结论:以物理性的缺氧2h-复氧1h的方式能诱导所培养的心肌细胞发生凋亡;XIAP能明显减少缺氧-复氧诱导新生大鼠心肌细胞的凋亡;XIAP抑制凋亡的效应与缺氧预处理抑制凋亡的效应相似。
Objective：To study whether X-linked inhibitor of apoptosis protein （XIAP） could suppress apoptosis which induce apoptosis of cardiomyocytes by transfected with pDsRed2- XIAP, inorder to search for new gene therapy for ischemic heart diseases. Method：The cardiocytes of neonate SD rat were cultured for four days. To set up fourstudy groups： （1）the XIAP group：pDsRed2-XIAP plasmid was transfected into cadiocytes by liposomes, after 48 hours, then making hypoxia for 2hs and reoxygenation for 1 h;（2）the preconditioning group ： firstly makingprecondition then carrying out 2 h of hypoxia and 1 h of reoxygenation; （3）the hypoxia/reoxygenation group：directly making cardiocytes hypoxia for 2h and reoxygenation for lh; （4）the normoxia group： to make cardiocytes incubate for 3h in CO2. Lastly,each group were detected apoptosis rates of myocardial cell by Annexin V FITC. The difference of each group was analyced by one-way ANOVA. Result： （1）pDsRed2-XIAP plasmid can be transfected into cardiomyocytes by liposomes. （2）Compared with the hypoxia/reoxygenation group, cardiomyocytes apoptosis rates in the XIAP group and the preconditioning group obviously decrease with statistics significance. （3）Cardiomyocytes apoptosis rate in the XIAP group and the pretreatment group are similar without statistics significance. Conclusion：Apoptosis of cardiomyocytes could be induced by hypoxia for 2 h and reoxygenation for 1 h XIAP. The cell apoptosis regulatory factor, can obviously reduce cardiomyocytes apoptosis induced by hypoxia/reoxygenation in newly-born rat. The effect of inhibiting apoptosis is similar between X-linked inhibitor of apoptosis protein and hypoxia-pretreat merit.
出处
《临床心血管病杂志》
CAS
CSCD
北大核心
2009年第11期853-855,共3页
Journal of Clinical Cardiology
基金
贵州省科学技术基金(No:黔科合J字[2007]2128)
关键词
心肌细胞
X染色体连锁凋亡抑制蛋白
预处理
心肌细胞凋亡
凋亡抑制
cardiomyoeytes
X-linked inhibitor of apoptosis protein
preconditioning
cardiomyocytes apoptosis
apoptosis suppress
