摘要
目的探讨核因子κB（NF—κB）在肝癌组织中的表达特点及其与肿瘤坏死因子α（TNF-α）、临床病理学特征的关系。方法以自身配对法收集手术后肝癌及其癌周组织各30份，以免疫组织化学法和酶联免疫吸附法检测肝癌和癌周组织中NF-κB的定位和表达，并以酶联免疫吸附法定量分析肝癌和癌周组织中TNFα的水平。样本均数间的比较先进行方差齐性检验，方差相等时进行t检验或方差分析；样本率之间的比较采用χ^2检验；等级资料的比较采用秩和检验；相关性分析采用Pearson相关分析。用Stata7．0统计软件包处理、分析数据。结果NF—κB蛋白在肝癌组织的胞核和胞质中均有表达，而对应的癌周组织中仅在胞质中有表达；NF—κB表达的阳性率肝癌组织中为100．0％，癌周组织为63．3％，χ^2=13．47，P〈0．01，差异有统计学意义。NF—κB表达强度：肝癌组织（＋）4例、（＋＋）10例、（＋＋＋）16例，癌周组织（-）11例、（＋）7例、（＋＋）9例、（＋＋＋）3例，肝癌组织中NF-κB的表达强度明显高于其对应的癌周组织，u=4．44，P〈0．01，差异有统计学意义。核蛋白NF—κB的表达水平，肝癌组织中大约是癌周组织的1．9倍，t=23．17，P〈0．01，差异有统计学意义。TNFα的表达水平在肝癌组织中大约是癌周组织的2．3倍，t=39．22，P〈0．01，差异有统计学意义。核蛋白NF—κB及TNFα的表达呈显著正相关，r=0．964，t=19．31，P〈0．01。肝癌组织中核蛋白NF—κB的表达水平与肿瘤的分化程度、肿瘤数目、肿瘤大小和HBsAg是否阳性未见明显的相关性，各组间的差异无统计学意义。结论TNFα可诱导NF-κB活化，进入细胞核内参与肝癌的发生和发展。
Objective To investigate the expression characteristics of nuclear factor kappa B （NF- κB） in hepatocellular carcinoma （HCC） tissues and its correlation with tumor necrosis factor alpha （TNF α） and clinical pathological features. Methods Thirty liver specimens from HCC patients were collected by self-control method. The localization and expression of NF- κB in HCC and their surrounding tissues were detected by immunohisto-chemistry and enzyme linked immunosorbent assay （ELISA）, respectively. And the levels of TNF in these tissues were analyzed by ELISA. Results The expressed NF- κB was localized in nucleus and cytoplasm in HCC, whereas only in cytoplasm in the surrounding tissues. The expression level and density of NF- κB in HCC tissues were obviously higher than those in the surrounding tissues （P 〈 0.01）, which was positively correlated with increased TNF α in HCC tissues （r = 0.964, P 〈 0.01）. No positive correlation was found between NF- κB expression and histological differentiation grade, number of tumor, size of tumor, and HBsAg positive （P 〉 0.05）. Conclusion The expression and localization of NF- κB in HCC tissues are obviously different from those in the surrounding normal liver tissues, and the level of nucleoprotein NF- κB in HCC tissues is correlated with expressed TNF α, suggesting that TNF α can activate NF- κB, the activated NF- κB then translocates to the nucleus and plays important role in the carcinogenesis of HCC.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2009年第11期843-846,共4页
Chinese Journal of Hepatology
关键词
癌
肝细胞
核因子ΚB
肿瘤坏死因子Α
相关性分析
Carcinoma, hepatocellular
Nuclear factor kappa B
Tumor necrosis factor alpha
Correlation analysis
