摘要
目的:观察蟾蜍灵对HL-60细胞活力和凋亡的影响,对NF-κB通路的作用,探讨NF-κB通路在蟾蜍灵诱导HL-60细胞凋亡过程中的作用。方法:锥虫蓝染色检测细胞活力;流式细胞仪技术检测细胞凋亡和周期分布;采用WesternBlot技术检测IKK的磷酸化。结果:蟾蜍灵以时间和剂量依赖性方式诱导HL-60细胞凋亡。24,48和72h抑制细胞活力的IC50浓度分别为26.3,7.8和2.0nmol/L。对照组HL-60细胞有pIKK表达,蟾蜍灵可诱导IKK的快速活化。NF-κB通路的特异性抑制剂Bay11-7082增强蟾蜍灵的凋亡诱导作用。结论:蟾蜍灵可诱导HL-60细胞凋亡,促进IKK活化与NF-κB通路调节相关,而且与NF-κB通路抑制剂有协同作用。
Objective ：To investigate the effects of bufalin on HL- 60 cell viability, apoptosis and NF- κB path- way activation, nehtods：Cell viability was tested by Trypan Blue Staining. Apoptosis was detected using flow cytometery assay. The expressions of pIKK and IKK were tested by using Western Blot. Results： Bufalin inhibited HL - 60 cell viability in a time - and dose - dependent manner. Concentrations of bufalin that inhibited 50% cell viability （IC50） for 24h,48h and 72h were 26.3,7.8 and 2.0 nmol/L,respectively. In the untreated control cells,IKK was basically phosphorylated. And phosphorylation level was transiently up - regulated after exposure to bufalin. Bay 11 - 7082, a NF- κB pathway specific inhibitor, enhanced bufalin induced -HL- 60 cell apoptosis. Conclusion ：NF- κB pathway might be involved in cell proliferation and survival,and might also affect apoptosis - induced effect of bufalin.
出处
《现代肿瘤医学》
CAS
2009年第11期2039-2042,共4页
Journal of Modern Oncology
基金
国家自然科学基金(编号:30700807)
辽宁省科学技术厅基金资助项目(编号:2004225004-11)
