摘要
背景:传统的羟基磷灰石强度低、孔隙度小、骨诱导性和传导性较差。因此,人们采用各种方法制备羟基磷灰石生物复合材料,以改进其性能。目的:观察丝素蛋白/羟基磷灰石复合材料对骨髓间充质干细胞生物特性的影响,并与常用的胶原海绵相比较。设计、时间及地点:重复测量观察及多样本观察实验,于2007-01/2008-06在苏州大学附属第一医院骨科实验室完成。材料:丝素蛋白/羟基磷灰石纳米材料由苏州大学材料学院李明忠教授研制提供,胶原海绵购买于上海其胜公司。方法:取SD大鼠股骨和胫骨分离提取骨髓间充质干细胞,进行培养,传代。取第3代骨髓间充质干细胞分别接种到丝素蛋白/羟基磷灰石复合材料及三维胶原海绵材料上进行共培养。未加材料,单独培养细胞,作为空白对照组。主要观察指标:应用倒置显微镜观察细胞生长情况;在培养第2,4,6,8天用MTT法测定细胞增殖情况及测定碱性磷酸酶活性。结果:①倒置显微镜显示骨髓间充质干细胞能在两种材料上良好地黏附、增殖和生长。②MTT法检测显示两种材料上的细胞增殖明显,碱性磷酸酶活性随培养时间延长而增加,均高于空白对照组。结论:丝素蛋白/羟基磷灰石复合材料显示出良好的生物相容性,并能促进细胞生长分化,其有望成为胶原组织工程支架材料很好的补充和替代。
BACKGROUND： Conventional hydroxyapatite is limited because of its low strength, small porosity, and poor osteoconduction. A novel scaffold, mixed silk fibroin with hydroxyapatite （SF/HA）, might be a promising choice. OBJECTIVE： To observe the effects of SF/HA scaffold on biocompatibility with the bone marrow mesenchymal stem cells （BMSCs）, and to compare with common collagen sponge. DESIGN, TIME AND SETTING： The study of repetitive measurement and multiple sample observation was performed at the laboratory of the Department of Orthopaedics, First Affiliated Hospital, Soochow University from January 2007 to June 2008. MATERIALS： SF/HA scaffold was supplied by Professor Li Ming-zhong, from the College of Material, Soochow University. Collagen sponge was purchased from Shanghai Qisheng. METHODS： Rat BMSCs were harvested from the femur and tibia, and then subcultured. BMSCs at the third passage were incubated on the both SF/HA and collagen sponge scaffold in vitro. Cells were cultured without scaffold as blank control group. MAIN OUTCOME MEASURES： The morphologic function of the BMSCs was observed by inverted microscope. The proliferation of the BMSCs on the scaffold and alkaline phosphatase （ALP） activity were measured by MTT assay at days 2, 4, 6 and 8. RESULTS： The BMSCs adhered, proliferated and grew perfectly on the both scaffolds under the inverted microscope. The evaluations of cellular proliferation and ALP activity were not influenced by the scaffolds. They were increased with culture time, and greater than control group. CONCLUSION： The SF/HA scaffold has good biocompatibility, can promote cell growth and differentiation, and can be a good supplement and substitute for collagen tissue engineering scaffold.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第42期8237-8240,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
