摘要
【目的】检测人β防御素-2(hBD-2)在正常子宫内膜、子宫内膜异位症(内异症)患者的在位和异位内膜组织中的表达,探讨hBD-2在内异症发病中的作用。【方法】选择25例内异症患者的卵巢异位内膜组织(异位内膜组)和相应的在位子宫内膜(在位内膜组),以及25例非子宫内膜异位症的子宫肌瘤患者(对照组)的子宫内膜组织。采用实时荧光定量PCR(Real-Time PCR)和原位杂交技术检测各组内膜组织中hBD-2的基因表达,以及hBD-2在内异症不同临床分期的异位内膜和在位内膜组织中的表达差异。【结果】异位内膜组内膜组织中hBD-2的基因表达水平显著高于在位内膜组和对照组(P<0.05)。在位内膜组和对照组子宫内膜组织中hBD-2的基因表达水平,差异无统计学意义(P>0.05)。异位内膜组中,hBD-2临床分期表达为Ⅰ期高于Ⅲ期和Ⅳ期、Ⅱ期高于Ⅲ期和Ⅳ期,差异有统计学意义(P<0.05)。【结论】异位内膜组织中hBD-2的高表达可能参与了内异症的发病。
[Objective] To investigate the expression of human β defensin-2 （hBD-2） in the eutopic and ectopic endometrium of women with ovarian endometriosis and explore the role of hBD-2 in the pathogenesis of endometriosis. [Methods] Twenty-five women with ovarian endometriosis and 25 controls were studied. Expression of hBD-2 was investigated quantitatively using real-time PCR and in-situ hybridization. Expression of hBD-2 was compared in the eutopic and ectopic endometrium of different clinical stage. [Results] hBD-2 gene expression was significantly stronger in the ectopic endometrium group than that in the eutopic endometrium group and the control group （P 〈 0.05）, but there were no significant difference between the control group and the eutopic endometrium group. In the ectopic endometrium group, hBD-2 expression in the first stage and second stage were obviously stronger than that in the third stage and forth stage （P 〈 0.05）, respectively. [Conclusion] Up-regulated expression of hBD-2 might play a pathogenic role in the formation of endometriosis.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2009年第6期747-752,共6页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省科技计划项目(2007B030502006)
