摘要
目的了解急性心肌梗死后梗死周边区心肌组织中HCN2和HCN4的mRNA和蛋白表达的动态变化。方法通过结扎大鼠左冠状动脉前降支建立急性心肌梗死模型,将成功建模的大鼠随机分为24 h组、1周组、2周组和4周组,同时于各时间点均设立假手术组,每组5只。于各时间点末取左心室梗死周边区心肌组织样本(假手术组取左心室相应部位的心肌组织),用逆转录聚合酶链反应检测HCN2和HCN4 mRNA的表达,用免疫组织化学和免疫印迹法检测HCN2和HCN4蛋白的表达。结果假手术组左心室心肌组织中存在HCN2和HCN4通道蛋白的表达,梗死周边区心肌组织中HCN2和HCN4的表达在梗死后24 h出现上升趋势,于梗死后1周表达达到峰值,之后逐渐下降,梗死后4周HCN2 mRNA和蛋白的表达仍高于对照组,而HCN4的表达已回落至假手术组水平。结论急性心肌梗死后左心室梗死周边缺血区心肌组织HCN2和HCN4通道蛋白的表达呈动态变化趋势,梗死后1周表达明显增高。
Ahn To investigate the dynamic changes of the expression of HCN2 and HCN4 mRNA and protein in infarcted border zones of post-AMI heart. Methods AMI model induced by the left anterior descending coronary artery （ LAD ） ligation in Sprague- Dawley rats, were randomly divided into 24 hours group, 1 -week group, 2-week group, 4- week group. Accordingly, the sham-operation group was established. Left ventricular infarcted border zone myocardial tissues were harvested at each time, meanwhile, corresponding myocardial tissues in sham-operated rats were harvested too. HCN2 and HCN4 mRNA and protein expression were measured by RT-PCR, immunohistochemistry and Western blotting. Results The expression of HCN2 and HCN4 mRNA and protein were detected in sham-operated rats. The ex- pression of HCN2 and HCN4 mRNA and protein underwent a dynamic change process. The expression of two of them ex- hibited an ascending tendency at 24 hours after AMI, reached peak value at 1 week after AMI, decreased gradually after that. But the expression of HCN2 channel was also increased compared with sham-operated group at 4 week after AMI, while there was no significant difference in HCN4. Conclusion Expression of HCN2 and HCN4 mRNA and protein in infarcted border zone myocardial tissues underwent a dynamic change process and reached peak value at 1 week after AMI.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2009年第9期719-722,共4页
Chinese Journal of Arteriosclerosis
关键词
心肌梗死
梗死周边区
HCN通道
Myocardial Infarction
Infarcted Border Zone
HCN Channel
