摘要
目的观察赤芍煎剂对急性胰腺炎（AP）大鼠的治疗效果及对NF-kB，INF-α，IL-6表达水平的影响。方法SD大鼠40只随机分为5组：正常对照组（A组）、AP模型组24h点（B组）、AP模型组72h点（C组）、赤芍煎剂治疗组24h点（D组）和赤芍煎剂治疗组72h点（E组），每组8只。采用工厂精氨酸腹腔内注射的方法诱发急性胰腺炎模型，造模后立即灌胃生理盐水（B，C组）、赤芍煎剂（D，E组），于造模后24h，72h时间点处死各组大鼠。采血检测血清淀粉酶；取胰腺组织分3份：HE染色行光镜检查、WesternBlot法检测NF-kB065蛋白表达、实时荧光定量PCR技术进行TNF-α、IL-6的mRNA定量分析。结果造模24h后与A组比较，B，D组大鼠血清淀粉酶均升高（P〈0．05）。D组与B组比较淀粉酶水平明显降低（P〈0．05）。造模后72h，E，C组淀粉酶水平均下降接近正常组水平（P〉0．05）。D，E组较同时间点B，C组的病理改变和病理学评分均明显减轻（P〈0．05）。Westernblotting结果显示，D，E组较同时间点B，C组NF-kB p65蛋白的表达明显减少[D组与B组相比，（0．07±0．006）vs．（O．71±0．029），P〈0．05；E组与C组相比，（0．18±0．014）vs（0．65±0．026），P〈0．05）]。实时荧光定量PCR结果显示：模型组TNF-α，IL-6mRNA的表达明显高于赤芍煎剂治疗组（P〈0．05）。结论赤芍煎剂对AP大鼠治疗作用的机制可能与其抑制NF-kB活化、在整体水平上抑制细胞因子基因表达、使机体免受过量细胞因子的损伤等因素有关。
Objective To observe the therapeutic effects of Radix Paeoniae Rubra decoction （RPRD） on acute pancreatitis （AP） in rats, and to study effects of RPRD on the expression of NF-kB, TNF-α and IL-6. Method Forty Sprague-Dawley （SD） rats were randomly divided into five groups： normal control group （group A）, AP model groups （group B, 24 h; group C,72 h） and RPRD treatment groups （group D, 24 h;group E,72 h）. There were 8 rats in each group. The AP models were induced by intraperitoneally injecting with L-arginine. After the model was established, rats of group B and group C were treated with intra-gastric instillation of NS, and rats in group D and group E with RPRD. At 24 and 72 hours after induction of AP, rats in each group were sacririced and blood samples were taken for the measurement of serum amylase. The expression of NF-kB p65 protein and TNF-α and IL-6 mRNA in the pancreas was detected by Western blotting and real-time quantitative PCR, respectively, and the pathological status of the pancreas was analyzed. Results The serum amylase level in group B and group D was obviously increased at 24 hours, and were significantly higher than that in group A （ P 〈 0.05） ; the level in group D was lower than that in group B （P 〈 0.05）. the level reduced at 72 hours, and there was no difference between group E and group C （ P 〉 0.05）. The pathological severity and the pathological score of pancreatic tissue in group D and group E at 24 hours and 72 hours were more lighter than those in group B and hours C （P 〈 0.05）. The expression levels of NF-kB p65 protein （Western blotting） in the pancreas of group D and group E were significantly lighter than those in group B and group C at both time points （group D compared with group B,（0.07 ±0.006） vs. （0.71 ± 0.029）, P 〈 0.05;group D compared with B, （0.07 ± 0.006） vs. （0.71 ±0.029）, P 〈0.05; group E compared with C, 0.18±0.014 vs. 0.65±0.026, P 〈0.05）. The results of real-time quantitative P
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2009年第11期1141-1145,共5页
Chinese Journal of Emergency Medicine
关键词
急性胰腺炎
赤芍煎剂
NF-KB
细胞因子
Acute pancreatitis
Radix Paeoniae Rubra decoction
Nuclear factor-kappaB
Cytokines
