摘要
目的:探求渐进窗口正交投影分析法对不同条件下决明子的色谱指纹图谱进行峰匹配的原理及依据。方法:样品用1.5 mol.L-1盐酸液加热水解,用氯仿回流提取,利用不同高效液相色谱仪、色谱柱,采用梯度洗脱技术进行HPLC分离分析。通过渐进窗口正交投影分析法对不同色谱条件下获得的决明子的HPLC指纹图谱进行谱间峰匹配。结果:得到了不同色谱条件下的决明子的特征指纹图谱。结论:采用渐进窗口正交投影分析法可以准确地进行不同指纹图谱间色谱峰的匹配。
Objective： To investigate the principle and basis about the chromatographic fingerprint peak matching of Semen Cassiae by the asymptotic window orthogonal projection analytical method. Method ： The samples of Semen Cassiae were hydrolyzed in the 1.5 mol · L-1 hydrochloride acid and then reflux extracted with chloroform. The chromatographic condition was that the HPLC was run on Agilent 1100 column, Zorbax Eclipse XDB-Cls （4. 6 mm ×250 mm, 5μm）. The protected column was Scienhome Cls column （3.0 mm ×20 mm, 5 μm） with acetonitrile-water （0. 1% phosphoric acid） as mobile phase in gradient elution at a flow rate of 1.0 mL ·min^-1. The detection wavelength and reference wavelength were 278 nm and 550 nm, respectively. Result： The characteristic fingerprint collection of illustrative plates of Semen Cassiae were obtained with different HPLC instruments and chromatographic columns. And the asymptotic window orthogonal projection analytical method was used to decide the chromatographic fingerprint peak matching of Semen Cassiae, promptly and prapidly. Conclusion ： The asymptotic window orthogonal projection analytical method can progress the matching of different fingerprint collection of illustrative plates peak matching well and truly.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第22期2876-2879,共4页
China Journal of Chinese Materia Medica
关键词
渐进窗口正交投影分析法
决明子
指纹图谱
峰匹配
asymptotic window orthogonal projection analytical method
Semen Cassiae
fingerprint collection of illustrative plates
peak matching
