摘要
目的:观察槲皮素(Quercetin,Que)对高糖状态下牛视网膜毛细血管周细胞(bovine retinal microvascular pericyte,BRPC)增殖和凋亡的影响。方法:用MTT比色法观察25,50,100μmol/LQue2,4,6d,对高糖(25mmol/L)培养BRPC增殖的影响;TUNEL法检测6d后Que对BRPC凋亡的影响。结果:高糖组较正常组吸光度值降低(P<0.01),槲皮素组较高糖组吸光度值升高(P<0.05),以高浓度槲皮素组吸光度值最高。TUNEL法检测显示:高糖状态下部分BRPC出现凋亡改变,高糖组较正常组凋亡率升高(P<0.01),槲皮素组较高糖组凋亡率降低(P<0.05),以高浓度槲皮素凋亡率最低。结论:Que可减轻高糖对BRPC增殖的抑制作用,减少高糖状态下BRPC的凋亡。
AIM：To investigate the effects of quercetin on the proliferation,apoptosis of bovine retinal microvascular pericyte（BRPC） cultured with the high glucose medium in vitro.·METHODS：Using 25mmol/L glucose to simulate high glucose condition,BRPCs were cultured with the high glucose medium and incubated with different concentrations of quercetin（25,50,100μmol/L） in the following experiments.Proliferation of BRPCs was assessed by MTT colorimetry at different time（2,4 and 6 days）.Cell apoptosis of BRPCs was detected by TUNEL straining after 6 days.·RESULTS：MTT assay showed that：compared with normal group,the absorbance value reduced in high glucose group（P〈0.01）;compared with high glucose group,the absorbance value increased in quercetin group（P〈0.05）;the absorbance value was highest in high concentration quercetin group.TUNEL assay showed that：a few cells appeared apoptosis;compared with normal group,the apoptosis rate increased in high glucose group（P〈0.01）;compared with high glucose group,the apoptosis rate reduced in quercetin group（P〈0.05）;the apoptosis rate was lowest in high concentration quercetin group.·CONCLUSION：High glucose has an inhibitory effect on the proliferation of BRPCs and quercetin can alleviate inhibitory effect.High glucose induces apoptosis of BRPCs and quercetin can inhibit apoptosis of BRPCs.
出处
《国际眼科杂志》
CAS
2009年第11期2078-2080,共3页
International Eye Science
基金
中国山东省青岛市科技局2006科技发展指导计划项目(No.K2d-87)~~
关键词
槲皮素
高糖
周细胞
增殖
凋亡
quercetin high glucose pericyte proliferation apoptosis
