摘要
目的观察T细胞特异转录因子T—bet／GATA-3在支气管哮喘中失衡表达，探讨黄芪和地塞米松对其不同的调节作用。方法40只雄性SPF级SD大鼠随机分为对照组、哮喘组、地塞米松组和黄芪组，20只雄性SPF级致敏SD大鼠脾脏中分离获得CD4^＋T细胞，苏木精-伊红染色观察气道病理改变；酶联免疫吸附试验（ELISA）检测大鼠血清中IL-4、IL-5、IFN-γ含量；逆转录-聚合酶链反应（RT-PCR）检测IL-4、IL-5、IFN-γ、T—bet和GATA-3mRNA表达；Western blot法检测T—bet和GATA-3蛋白表达。结果肺组织中嗜酸粒细胞（EOS）、淋巴细胞、管壁面秽支气管管腔内周长（WA／Pi）和支气管平滑肌面积／支气管管腔内周长（ASM／Pi）哮喘组比对照组明显增多或增厚（P均〈0．01），地塞米松组和黄芪组明显低于哮喘组（P均〈0．01）；血清中IL-4和IL-5含量，哮喘组高于对照组（P均〈0．01），地塞米松组和黄芪组均低于哮喘组（P均〈0．01），血清中IFN-γ含量哮喘组远低于对照组（P〈0．01），地塞米松组低于对照组（P〈0．01），但黄芪组明显高于哮喘组（P〈0．01）。在大鼠肺组织和致敏大鼠脾CD4^＋T细胞中，IFN-γ mRNA、T-bet mRNA和蛋白表达哮喘组明显低于对照组（P均〈0．01），地塞米松组与哮喘组相似（P〉0．05），黄芪组与对照组相似，但明显高于哮喘组（P〈0．01）；IL4和IL-5mRNA、GATA-3mRNA和蛋白表达，哮喘组异常高于对照组（P〈0．01），地塞米松组和黄芪组均明显低于哮喘组（P〈0．01）；肺组织中T—bet／GATA-3蛋白表达量比值，哮喘组明显低于对照组（P〈0．01），地塞米松组与哮喘组相近（P〉0．05），黄芪组与对照组的比值相近（P〉0．05），明显高于哮喘组和地塞米松组（P均〈0．01）。结论T细胞特异转录因子T—bet／GATA-3在哮喘中失衡表达，黄芪抑制哮喘气道炎症可能通过双向调�
Objective To investigate imbalanced T cell-specific transcription factors T-bet and GATA-3 in asthmatic rats, and to identify the different effects of astragalus and dexamethasone on them. Methods Forty male SD rats were randomly divided into a control group, an asthmatic group, a dexamethasone group, and an astragalus group, and were observed the change of airway histology. Two weeks after immunization, CD4^＋ T cells were obtained from singled-cell suspension of spleen （after lysis of RBCs）. The concentrations of IL-4, IL-5, IFN-γ in serum were measured by ELISA. The mRNA expressions of IL-4, IL-5, IFN-γ, T-bet and GATA-3 in the lungs and CD4 ^＋ T cells were detected by RT-PCR, and the protein expressions of T-bet and GATA-3 in the lungs and CD4^＋ T cells were detected by Western blot respectively. Results In the asthmatic group, the numbers of eosinophils and lymphocytes, the thicknesses of WA/Pi and ASM/Pi were （25.70 ± 2.50）/mm^2, （44.80 ± 5.69 ）/mm^2, （ 12. 15 ± 1.47 ） mm, （6.82 ± 0.91 ） mm, and those of the control group were （2.40 ± 1.27 ）/mm^2, （8.90 ± 1.97）/mm^2, （6.49 ±1.57） mm, （2.75 ±0. 62） mm, and those of the dexamethasone group were （6.70 ± 1. 89）/mm^2, （15. 80± 3.12） /mm^2, （8.02±1.35）mm, （3.38±0.65）mm, and those of the astragalus group were （11.20 ± 2.97）/mm^2 , （ 19.80 ± 4.59） /mm^2, （ 8.86 ± 0.96） mm, （ 3.96 ± 0. 61 ） mm. There were significant differences between the asthmatic group and the control group, the asthmatic group and the dexamethasone group, and the astragalus group （ P 〈 0.01 ）. In the asthmatic group, the concentrations of IL-4, IL-5, IFN- γ in serum were （35.23 ±8.02） pg/ml, （32.03 ±3.99） pg/ml, （ 16.73 ±5.15） pg/ml, and those of the control group were （ 14.48 ± 1.68 ） pg/ml, （ 14.23 ± 0.97 ） pg/ml, （64.55 ± 14.04） pg/ml, and those of the dexamethasone group were （ 14.97±1.09） pg/ml, （ 16.13 ±2.36） pg/ml, （ 16.69 ± 4.05 ） pg/ml, those of
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2009年第10期907-913,共7页
Chinese Journal of Microbiology and Immunology
关键词
哮喘
转录因子
T淋巴细胞
黄芪
地塞米松
Asthma
Transcription factors
T lymphocyte
Astragalus membranaceus
Dexamethasone
